Biochemical and biomedical engineering
Viral and non-viral gene/drug delivery; virus-like particle, viral vector and biopolymer synthesis; animal cell culture; metabolic analysis; synthetic biology; systems biology.
Heterogeneous catalysis and process engineering as applied to environmental and biochemical engineering problems.
Nanotechnology, biomaterials, biomedical engineering, drug and gene delivery, colloid and surface science, interfacial engineering, polymer and biopolymer synthesis.
Bioprocess development for biomanufacturing, genetic/protein engineering, metabolic engineering, molecular biology, microbial biotechnology, gene expression, recombinant protein production, fermentation, downstream processing, protein folding.
Development of self-assembled polymeric nanoparticles for the delivery of conventional small molecule therapeutic agents. Our technology aims to improve the therapeutic index of currently available drugs by optimizing their efficacy and toxicity, and to improve the solubility and stability of drug entities that have been stalled in preclinical trials because of suboptimal biochemical properties. Also specialize in designing and optimizing the processes in nanoparticle manufacturing, which include particle formation, collection, drying and sterilization.
The underlying hypothesis driving this research program is that an under-standing of transport in the cytoplasm and cellular microenvironment is required for the full realization of host integrated tissue prosthesis. The primary tool used for this work is Multiphoton Laser Scanning Microscopy (MP-LSM). The MP technique uses non-linear optical effects to achieve 3D imaging. Basic principles in bio-chemical engineering are applied with MP microscopy for applications including light induced drug activation, MP-photobleaching analysis of trans-port, and 3D live-cell time course imaging.
Research interests focus on protein and biomimetic engineering including separation and purification of enzymes, use of enzymes in non-conventional media, reactor design for biotransformations in non-conventional media, enzyme immobilization, immobilized enzyme kinetics, biosensor development, protein thermodynamics and stability and analysis of coupled enzymatic reactions. Research in the area of environmental engineering includes bioremediation of contaminated soils, wastewater treatment and the development of separation processes for treatment of waste effluents.
Enzyme reaction engineering; wood pulp characterization; food processing and waste reutilization; membrane filtration processes; polymeric membrane barrier properties.
Nano-structured systems from templating process, enhanced drug delivery systems, Atom Transfer Radical Polymerization (ATRP), stimuli responsive polymeric systems, polymer-surfactant interactions, associative polymers for environmentally friendly coatings, magnetic nanoparticles for novel separation processes.