The Zeiss LSM 510 Meta Confocal Microscope comes with the following features:

  • 7 laser lines to select from
    • UV 405nm at 25mW (for violet excitation of dyes such as BFP, DAPI, Hoechst, AMCA)
    • Tunable Argon 458/477/488/514nm at 30mW (458, 477 and 488 for blue excitation of dyes such as GFP, CFP, FITC, CY2; 514 for green excitation of dyes)
    • HeNe1 543nm at 1.0mW (as well as Argon 514 for green excitation of dyes such as Rhodamine, CY3, YFP, Propidium Iodide, Alexa 546)
    • HeNe2 633nm at 5.0mW (for red excitation for dyes such as CY5)
  • 5 objective lenses available
    • C-Apochromat 10x/0.45 W
    • Plan-Neofluar 25x/0.8 Imm corr DIC
    • C-Apochromat 40x/1.2 W corr
    • Plan-Neofluar 40x/1.3 Oil DIC
    • Plan-Apochromat 63x/1.4 Oil DIC
  • 2 PMTs (photomultiplier tubes)
  • 1 Meta detector (polychromatic detector, can be used as 3rd PMT)

What the Zeiss LSM 510 Meta Confocal microscope can do for you:

  • Z sectioning (Z-stacking) and 3D reconstruction
  • Time series for cellular events
  • Co-localization of multiple proteins and/or dyes

Imaging Software used to process raw images generated on the Confocal

  • Zen 2009 software can be downloaded for free for all users
     

For any additional information please contact Mishi Groh at:
mishi.groh@uwaterloo.ca or ext. 32375.