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Information for

Marc Aucoin

Associate Professor & Academic Director WatPD-Engineering

Marc AucoinDegrees

  • BASc, University of Waterloo
  • MASc, University of Waterloo
  • PhD, Ecole Polytechnique de Montréal

Research interests

Baculovirus Bioprocessing

We are currently actively pursuing different ways of characterizing and manipulating baculoviruses to enhance their use as vectors. Is there a need? We believe so. That is also why we are actively participating in the discussions on the development of a standard reference material for baculoviruses. One major aspect that we are investigating on this front is the use of membrane chromatography to better the downstream processing of these vectors.

Biologic Production in Insect Cells using Baculovirus Expression Vectors

One of our biggest veins of this research is to try and gain a better understanding of competition between vectors when multiple vectors are used to produce a single product. How best to look at this problem? We are devising means based on synthetic biology to help us address some of these issues. We are also interested in the level of post-translational modification that insect cells are able to perform. Not only are we interested in glycosylation but also the less investigated process of myritoylation.

Cell Culture Monitoring, Modeling and Simulation – from metabolomics to on-line spectroscopy

We are at a point where we can collect huge amounts of data for our processes but do we use it to it's full advantage?  The answer to this is that even though we can amass a lot of information for our cultures we still do not know what to do with it. We are very interested in this aspect. One area of interest is the wider use of 1D-1H NMR metabolomics in cell culture. Does the increase in information gathered lead to more robust processes?”

Monoclonal Antibody Production and Modification

A billion dollar industry that is based on a therapeutic molecule that is inherently a population of molecules with different glycosylation patterns. Can we create processes that will enhance the monodispersity of the product? This is what we are trying to do. Not only are we interested in insect cells, but we are also interested in more traditional cell lines like the Chinese Hamster Ovary (CHO) cells. These cells continue to be the most widely used and still require a significant amount of study to fully understand how they do what they do best for us – make therapeutic proteins.

Selected references

  • Bradley RM, Marvyn PM, Aristizabal Henao JJ, Mardian EB, George S, Aucoin MG, Stark KD, Duncan RE (2015) Acylglycerophosphate acyltransferase 4 (AGPAT4) is a mitochondrial lysophosphatidic acid acyltransferase that regulates brain phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol levels. BBA - Molecular and Cell Biology of Lipids, 1851(12):1566–1576 DOI: 10.1016/j.bbalip.2015.09.005

  • Blondeel EJM, Braasch K, McGill T, Chang D, Spearman M, Butler M, Aucoin MG (2015) Tuning a MAb Glycan Profile in Cell Culture: Supplementing N-acetylglucosamine to Favour G0 Glycans without Compromising Productivity and Cell Growth. Journal of Biotechnology, 214:105–112 DOI: 10.1016/j.jbiotec.2015.09.014

  • Sokolenko S, Aucoin MG (2015) A correction method for systematic error in 1H-NMR cell culture data validated through stochastic time-course simulation. BMC Systems Biology,  9:51 DOI 10.1186/s12918-015-0197-4

  • Thompson CM, Petiot E, Mullick A, Aucoin MG, Henry O, Kamen AA (2015) Critical assessment of influenza VLP production in Sf9 and HEK293 expression systems. 15:31 DOI: 10.1186/s12896-015-0152-x

  • George S, Jauhar AM, Mackenzie J, Kieβlich S, Aucoin MG (2015) Temporal Characterization of Protein Production Levels from Baculovirus Vectors Coding for GFP and RFP Genes under Non-Conventional Promoter Control. 112(9):1822–1831 DOI: 10.1002/bit.25600

  • Yen S, McDonald JAC, Schroeter K, Oliphant K, Sokolenko S, Blondeel EJM, Allen-Vercoe E, Aucoin MG (2015) Metabolomic Analysis of Human Fecal Microbiota: A Comparison of Feces-Derived Communities and Defined Mixed Communities. 14(3):1472-82 DOI: 10.1021/pr5011247.

  • Yen S, Sokolenko S, Manocha B, Patras A, Daynouri-Pancino F, Blondeel E, Sasges M, Aucoin MG (2014) Treating Cell Culture Media with UV Irradiation against Adventitious Agents: Minimal Impact on CHO Performance. Biotechnology Progress, 30(5): 1190–1195

  • Sokolenko S, Blondeel EJM, Azlah N, George B, Schulze S, Chang D, Aucoin MG (2014) Profiling Convoluted 1D-1H-NMR Spectra: A Plackett-Burman Approach for Assessing Quantification Error of Metabolites Complex Mixtures with Application to Cell Culture 3330-3337

  • Srirangan K, Akawi L, Liu X, Westbrook A, Moo-Young M, Chou CP (2013) Manipulating the Sleeping Beauty Mutase Operon for the Production of 1-Propanol in Engineered Escherichia coli. Biotechnology for Biofuels, 6:139 DOI:10.1186/1754-6834-6-139

Affiliation: 
University of Waterloo