Mass spectral imaging: application to pathology and biomarker discovery
Dr. Bindesh Shrestha
Location: C2-361 (Reading Room)
Host: J. Pawliszyn
Abstract: Since the introduction of mass spectrometry imaging (MSI) in the late 1990s, the technique has been gaining utility in clinical research, omics, and pharma research. In the last few years, many ambient ionization techniques have been developed to ionize molecules directly off the tissue, such as desorption electrospray ionization (DESI). DESI is a complimentary direct sampling approach to the traditional matrix assisted laser desorption/ionization (MALDI). Combing DESI and MALDI, on a single instrument platform with integrated ion mobility separation and robust informatics tool, provides a significant technological advancement. This allows for wider molecular coverage, accurate identification, and flexible workflows. In this presentation, we will discuss the principle of MS imaging using MALDI and DESI ionization, applied to a variety of biomedical samples with focus on disease pathology and biomarker discovery.