Tumor associated carbohydrate antigens: synthetic chemistry and molecular modelling studies
Dr. France-Isabelle Auzanneau
Thursday, September 25, 2014
EIT-2053 (Main Link Room)
Abstract: A combination of synthetic carbohydrate chemistry and molecular modeling experiments are used to design anti-tumor vaccines based on the tumor associated carbohydrate antigens (TACAs) dimeric Lex (dimLex) and LeaLex. While it has been shown that these TACAs displayed internal epitopes that are only found at the surface of tumor cells, the non-reducing end trisaccharides (Lex and Lea) are also expressed on non-cancerous tissues. Thus, using the hexasaccharides as vaccine candidates carries the inherent risk of triggering an auto-immune response targeted against the terminal Lex and Lea antigens. To avoid such cross-reactivity, our program aims at characterizing internal epitopes displayed by the hexasaccharides as well as searching for analogues of the hexasaccharides that would not contain the Lex or Lea trisaccharides at the reducing end but still retain the internal epitopes found in the hexasaccharides. We will describe the synthesis of hexasaccharide vaccine candidates and that of fragments and analogues of these TACAs. We will also report our findings on the conformational behavior of the dimLex and LeaLex TACAs in solution.