Comparison of Three Competing Dynamic Force Spectroscopy Models to Study Binding Forces of Amyloid-β 1-42

TitleComparison of Three Competing Dynamic Force Spectroscopy Models to Study Binding Forces of Amyloid-β 1-42
Publication TypeJournal Article
Year of Publication2014
AuthorsHane, F., S. J. Attwood, and Z. Leonenko
JournalSoft Matter
Volume10
Start Page1924
Issue12
Pagination1924-1930
Date Published01/2014
Abstract

We performed single molecule dynamic force spectroscopy experiments to study the dimerization of two amyloid-β (1–42) peptides and compared three different theoretical models used to fit experimental data: Bell–Evans, Dudko–Hummer–Szabo, and Friddle–De Yoreo. Using these models we extracted values of the dissociation rate at zero force, k0, and height and the width of the energy barrier, ΔG and xβ. We show the importance of including the effect of the linker molecule. All three models corrected for the linker effect give comparable results for xβ and show more discrepancy for k0 and ΔG values, ΔG parameter correlates well between Dudko–Hummer–Szabo and Friddle–De Yoreo models but differs for the Bell–Evans model.

URLhttp://pubs.rsc.org/en/Content/ArticleLanding/2014/SM/c3sm52257a#!divAbstract
DOI10.1039/C3SM52257A
Refereed DesignationRefereed
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