Effect of Cholesterol and Amyloid-β Peptide on Structure and Function of Mixed-Lipid Films and Pulmonary Surfactant BLES: An Atomic Force Microscopy Study

TitleEffect of Cholesterol and Amyloid-β Peptide on Structure and Function of Mixed-Lipid Films and Pulmonary Surfactant BLES: An Atomic Force Microscopy Study
Publication TypeJournal Article
Year of Publication2010
AuthorsHane, F., E. Drolle, and Z. Leonenko
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume6
Start Page808
Issue6
Pagination808-814
Date Published12/2010
Keywordsamyloid-beta, atomic force microscopy, cholesterol, lipid, nanoscale structure, protein, pulmonary surfactant, surfactant dysfunction
Abstract

Pulmonary surfactant forms a thin molecular film inside mammalian lung alveoli and lowers the surface tension of the air/fluid interface to reduce the work of breathing. Upon compression functional surfactant forms characteristic multilayer structures, which indicate surfactant surface activity.We showed that cholesterol adversely affects both structural and surface-active properties of BLES surfactant and DPPC/DOPG lipid films. Incorporation of small concentrations of fibril-forming peptide amyloid-β 1-40 helps to counteract the distractive effect of cholesterol by improving characteristic multilayer formation that occurs upon compression. In contrast to many negative effects of amyloid-forming peptides reported earlier, we report a positive effect of amyloid-β peptide on surfactant function, which may aid in the designing of novel surfactant formulations.

URLhttp://www.nanomedjournal.com/article/S1549-9634(10)00155-3/abstract
DOI10.1016/j.nano.2010.05.001
Refereed DesignationRefereed
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