|Title||Effect of Cholesterol and Amyloid-β Peptide on Structure and Function of Mixed-Lipid Films and Pulmonary Surfactant BLES: An Atomic Force Microscopy Study|
|Publication Type||Journal Article|
|Year of Publication||2010|
|Authors||Hane, F., E. Drolle, and Z. Leonenko|
|Journal||Nanomedicine: Nanotechnology, Biology, and Medicine|
|Keywords||amyloid-beta, atomic force microscopy, cholesterol, lipid, nanoscale structure, protein, pulmonary surfactant, surfactant dysfunction|
Pulmonary surfactant forms a thin molecular film inside mammalian lung alveoli and lowers the surface tension of the air/fluid interface to reduce the work of breathing. Upon compression functional surfactant forms characteristic multilayer structures, which indicate surfactant surface activity.We showed that cholesterol adversely affects both structural and surface-active properties of BLES surfactant and DPPC/DOPG lipid films. Incorporation of small concentrations of fibril-forming peptide amyloid-β 1-40 helps to counteract the distractive effect of cholesterol by improving characteristic multilayer formation that occurs upon compression. In contrast to many negative effects of amyloid-forming peptides reported earlier, we report a positive effect of amyloid-β peptide on surfactant function, which may aid in the designing of novel surfactant formulations.