Effect of Cholesterol on the Physical Properties of Pulmonary Surfactant Films: Atomic Force Measurements Study

TitleEffect of Cholesterol on the Physical Properties of Pulmonary Surfactant Films: Atomic Force Measurements Study
Publication TypeJournal Article
Year of Publication2006
AuthorsLeonenko, Z., E. Finot, V. Vassiliev, and M. Amrein
JournalUltramicroscopy
Volume106
Start Page687
Issue8-9
Pagination687-694
Date Published04/2006
Keywordsadhesion forces, atomic force microscopy, cholesterol, phospholipids
Abstract

Atomic force measurements were performed on supported pulmonary surfactant (PS) films to address the effect of cholesterol on the physical properties of lung surfactant films. We recently found that cholesterol in excess of a physiological proportion abolishes surfactant function, and is the reason that surfactant fails to lower the surface tension upon compression. In this study, we investigated how the loss of mechanical stability observed earlier is related to the local mechanical properties of the film by local force measurements. The presence of 20% of cholesterol in bovine lipid extract surfactant (BLES) resulted in a decrease of the observed adhesive interaction, and an increase in rigidity of the film. We discuss the implication the increased rigidity might have on the functional failure of PS.

URLhttp://www.sciencedirect.com/science/article/pii/S0304399106000350
DOI10.1016/j.ultramic.2006.02.007
Refereed DesignationRefereed
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