Effect of Surfaces on Amyloid Fibril Formation

TitleEffect of Surfaces on Amyloid Fibril Formation
Publication TypeJournal Article
Year of Publication2011
AuthorsMoores, B., E. Drolle, S. Attwood, J. Simons, and Z. Leonenko
JournalPLoS ONE
Volume6
Start Pagee25954
Issue10
Date Published10/2011
Abstract

Using atomic force microscopy (AFM) we investigated the interaction of amyloid beta (Ab) (1–42) peptide with chemically modified surfaces in order to better understand the mechanism of amyloid toxicity, which involves interaction of amyloid with cell membrane surfaces. We compared the structure and density of Ab fibrils on positively and negatively charged as well as hydrophobic chemically-modified surfaces at physiologically relevant conditions. We report that due to the complex distribution of charge and hydrophobicity amyloid oligomers bind to all types of surfaces investigated (CH3, COOH, and NH2) although the charge and hydrophobicity of surfaces affected the structure and size of amyloid deposits as well as surface coverage. Hydrophobic surfaces promote formation of spherical amorphous clusters, while charged surfaces promote protofibril formation. We used the nonlinear Poisson-Boltzmann equation (PBE) approach to analyze the electrostatic interactions of amyloid monomers and oligomers with modified surfaces to complement our AFM data.

URLhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0025954
DOI10.1371/journal.pone.0025954
Refereed DesignationRefereed
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