|Title||Metal Particle-Enhanced Fluorescent Immunoassays on Metal Mirrors|
|Publication Type||Journal Article|
|Year of Publication||2007|
|Authors||Matveeva, E., I. Gryczynski, A. Barnett, Z. Leonenko, J. Lakowicz, and Z. Gryczynski|
|Keywords||fluorescence immunoassay, metal-enhanced fluorescence, nano-size metal particles, plasmonics, silver island films|
We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.