Metal Particle-Enhanced Fluorescent Immunoassays on Metal Mirrors

TitleMetal Particle-Enhanced Fluorescent Immunoassays on Metal Mirrors
Publication TypeJournal Article
Year of Publication2007
AuthorsMatveeva, E., I. Gryczynski, A. Barnett, Z. Leonenko, J. Lakowicz, and Z. Gryczynski
JournalAnalytical Biochemistry
Volume363
Start Page239
Issue2
Pagination239-245
Date Published04/2007
Keywordsfluorescence immunoassay, metal-enhanced fluorescence, nano-size metal particles, plasmonics, silver island films
Abstract

We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.

URLhttp://www.sciencedirect.com/science/article/pii/S0003269707000462
DOI10.1016/j.ab.2007.01.030
Refereed DesignationRefereed
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