Nanoscale Electrostatic Domains in Cholesterol-Laden Lipid Membranes Create a Target for Amyloid Binding

TitleNanoscale Electrostatic Domains in Cholesterol-Laden Lipid Membranes Create a Target for Amyloid Binding
Publication TypeJournal Article
Year of Publication2012
AuthorsDrolle, E., R. M. Gaikwad, and Z. Leonenko
JournalBiophysical Journal
Volume103
Start PageL27
Issue4
PaginationL27-L29
Date Published08/2012
Abstract

Amyloid fibrils are associated with multiple neurodegenerative disorders, such as Alzheimer’s disease. Although biological membranes are involved in fibril plaque formation, the role of lipid membrane composition in fibril formation and toxicity is not well understood. We investigated the effect of cholesterol on the interaction of model lipid membranes with amyloid-β peptide (Aβ). With atomic force microscopy we demonstrated that binding of Aβ (1–42) to DOPC bilayer, enriched with 20% cholesterol, resulted in an intriguing formation of small nonuniform islands loaded with Aβ. We attribute this effect to the presence of nanoscale electrostatic domains induced by cholesterol in DOPC bilayers. Using frequency-modulated Kelvin probe force microscopy we were able to resolve these nanoscale electrostatic domains in DOPC monolayers. These findings directly affect our understanding of how the presence of cholesterol may induce targeted binding of amyloid deposits to biomembranes. We postulate that this nonhomogeneous electrostatic effect of cholesterol has a fundamental nature and may be present in other lipid membranes and monolayers.

URLhttp://www.sciencedirect.com/science/article/pii/S0006349512007886
DOI10.1016/j.bpj.2012.06.053
Refereed DesignationRefereed
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