Surface Plasmon Resonance Imaging to Study the Molecular Mechanism of Alzheimer's Disease

TitleSurface Plasmon Resonance Imaging to Study the Molecular Mechanism of Alzheimer's Disease
Publication TypeConference Proceedings
Year of Conference2016
AuthorsOllagnier, A., E. Finot, E. Drolle, and Z. Leonenko
Conference NameALLSENSORS 2016: The First International Conference on Advances in Sensors, Actuators, Metering and Sensing
Volume1
Edition1
Date Published04/2016
PublisherCurran Associates, Inc.
Conference LocationVenice, Italy
ISBN978-1-61208-523-4
Abstract

We have developed a state-of-the-art surface plasmon resonance imaging (SPRi) apparatus combined with microfluidics and used it to detect the binding of amyloid beta peptides to the lipid membrane, an interaction known to be involved in the molecular mechanism of amyloid toxicity in Alzheimer’s disease, and to study protective effect of melatonin. Kinetics of binding was measured, and quantitative analysis was developed in order to detect melatonin partitioning into and amyloid binding to the membrane. We provided for the first time clear experimental evidence that the presence of melatonin in supported lipid membrane prevents the amyloid peptide from binding to the lipid membrane, thus gaining insight into the molecular mechanism of melatonin protection against amyloid toxicity in Alzheimer’s disease.

URLhttp://toc.proceedings.com/30342webtoc.pdf
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