250 kHz, 1.5 µm resolution SD-OCT for in-vivo cellular imaging of the human cornea

Title250 kHz, 1.5 µm resolution SD-OCT for in-vivo cellular imaging of the human cornea
Publication TypeJournal Article
Year of Publication2018
AuthorsTan, B., Z. Hosseinaee, L. Han, O. Kralj, L. Sorbara, and K. Bizheva
JournalBiomedical Optics Express
Keywordsalgorithm, Article, Biological tissues, Bowman membrane, bright field microscopy, broadband dielectric spectroscopy, cell structure, Cellular automata, Cellular imaging, Cellular structure, Coherent light, confocal microscopy, cornea cell, cornea disease, cornea epithelium, cornea stroma, cornea tissue, Corneal epithelium, Diagnosis, diagnostic imaging, eye movement, fiber optics, fluorescence imaging, Fourier transformation, head movement, histology, human, human tissue, Image processing, Imaging resolutions, live cell imaging, morphometry, Non-invasive diagnostics, normal human, optic nerve, optical resolution, Optical tomography, refraction index, signal noise ratio, slit lamp microscopy, Spectral domain optical coherence tomographies, spectral domain optical coherence tomography, ultrahigh resolution optical coherence tomography, Volumetric Imaging

We present the first spectral domain optical coherence tomography (SD-OCT) system that combines an isotropic imaging resolution of 1.5 µm in biological tissue with a 250 kHz image acquisition rate, for in vivo non-contact, volumetric imaging of the cellular structure of the human cornea. OCT images of the healthy human cornea acquired with this system reveal the cellular structure of the corneal epithelium, cellular debris and mucin clusters in the tear film, the shape, size and spatial distribution of the sub-basal corneal nerves and keratocytes in the corneal stroma, as well as reflections from endothelial nuclei. The corneal images presented here demonstrate the potential clinical value of the new high speed, high resolution OCT system for non-invasive diagnostics and monitoring the treatment of corneal diseases. © 2018 Optical Society of America.