Cytotoxic and inflammatory effects of contact lens solutions on human corneal epithelial cells in vitro

TitleCytotoxic and inflammatory effects of contact lens solutions on human corneal epithelial cells in vitro
Publication TypeJournal Article
Year of Publication2018
AuthorsOh, S., D. McCanna, L. Subbaraman, and L. Jones
JournalContact Lens and Anterior Eye
KeywordsAlamarBlue, Article, Borate buffer, buffer, cell culture, cell metabolism, cell proliferation, cell survival, cell viability, Cells, concentration response, contact lens solution, Contact Lens Solutions, controlled study, cornea cell, cornea epithelium, Corneal, Corneal epithelial cells, Cultured, cytokine, cytokine release, Cytokines, cytotoxicity, drug effect, electrochemiluminescence, Epithelium, epithelium cell, flow cytometry, HCE cell line (cornea epithelium), human, human cell, Humans, in vitro study, inflammation, interleukin 1beta, interleukin 6, interleukin 8, metabolism, pathology, phosphate buffer, poly(hexamethylenebiguanide), priority journal, resazurin assay, tumor necrosis factor, unclassified drug, Viability

Purpose: To ascertain the effect that four contact lens (CL) multipurpose solutions (MPS) have on the viability and release of pro-inflammatory cytokines from human corneal epithelial cells (HCEC). Methods: HCEC were exposed to four different MPS at various concentrations for 18 hours. The cells were also exposed to phosphate buffer, borate buffer, and PHMB. The cell viability was evaluated using the alamarBlue assay. The release of pro-inflammatory cytokines was measured using a Multiplex electrochemiluminescent assay. Results: MPS-A, MPS-B and MPS-C all reduced cell metabolic activity p 0.05. Exposing the cells to borate buffer and PHMB caused an increase in the release of TNF-α p < 0.05. Conclusions: This investigation demonstrates that at different concentration levels, several of the MPS tested showed a decrease in viability and an increase in the release of inflammatory cytokines from HCEC. The borate buffer component as well as PHMB appears to contribute to this pro-inflammatory reaction. © 2017 British Contact Lens Association