Determination of the release of PEG and HPMC from nelfilcon A daily disposable contact lenses using a novel in vitro eye model

TitleDetermination of the release of PEG and HPMC from nelfilcon A daily disposable contact lenses using a novel in vitro eye model
Publication TypeJournal Article
Year of Publication2018
AuthorsPhan, C. - M., H. Walther, R. Smith, D. Riederer, C. Lau, K O. K. O. Lorenz, L. Subbaraman, and L. Jones
JournalJournal of Biomaterials Science, Polymer Edition
Keywordsarea under the curve, Article, chromatography, contact lens, Contact lenses, Controlled drug delivery, Disposable contact lens, Drug delivery, drug delivery system, drug release, Eye, eye model, flow rate, HPMC, hyaluronic acid, hydrogel, Hydrogel contact lens, Hydroxypropyl methylcellulose, hydroxypropylmethylcellulose, in vitro study, kinetics, lacrimal fluid, lens, Liquid chromatography, Liquid chromatography - mass spectrometries, liquid chromatography-mass spectrometry, macrogol, mass spectrometry, nelfilcon a, PEG, Phosphate-buffered salines, Physiological models, Polyethylene glycols, priority journal, retention time, Surface active agents, Targeted drug delivery, tear flow, Wetting, wetting agent, Wetting agents

The traditional method to measure release of components from CLs is a vial containing a static volume of PBS (phosphate buffered saline). However, this model does not simulate physiologically relevant tear volume and natural tear flow, air exposure, and mechanical rubbing. These factors can significantly impact release kinetics. We have developed an in vitro eye model (OcuFlow) that simulates these parameters. The aim of the study was to measure the release of PEG (polyethylene glycol), and HPMC (hydroxypropyl methylcellulose) from a daily disposable hydrogel contact lens material (nelfilcon A; Dailies AquaComfort PLUS; DACP;) over 24 hrs using the OcuFlow platform. The elution of PEG and HPMC from DACP lenses was analyzed using LCMS (liquid chromatography mass spectrometry). The release of all wetting agents from the lenses followed a burst release pattern, which occurred within the first 1.5 hrs (P < 0.05). The release of PEG was greater than that of HPMC (P < 0.05). The amount of PEG and HPMC released at any given time was less than 1% of the amount in the blister pack solution. Our results suggest that HPMC and PEG are rapidly released from the CL. © 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.