A rapid extraction method to quantify drug uptake in contact lenses

TitleA rapid extraction method to quantify drug uptake in contact lenses
Publication TypeJournal Article
Year of Publication2018
AuthorsPhan, C. - M., S. Weber, J. Mueller, A. Yee, and L. Jones
JournalTranslational Vision Science and Technology
Volume7
KeywordsArticle, balafilcon A, comfilcon B, contact lens, controlled study, copolymer, Drug delivery, drug delivery system, Drug uptake, etafilcon a, eye surgery, hilafilcon B, hydrogel, incubation time, Ketotifen fumarate, lotrafilcon B, Moxifloxacin, Natamycin, nelfilcon a, nesofilcon A, senofilcon A, spectral sensitivity, timolol, Timolol maleate, ultraviolet spectrophotometry, unclassified drug
Abstract

Purpose: To develop a simple extraction procedure to quantify the uptake of four topical ocular pharmaceutical drugs into contact lenses (CLs). Methods: Four silicone hydrogel (SH) CLs (balafilcon A, senofilcon A, lotrafilcon B, comfilcon B) and four conventional hydrogel (CH) CLs (nesofilcon A, hilafilcon B, nelfilcon A, etafilcon A) were evaluated. The drugs studied were natamycin, moxifloxacin, timolol maleate, and ketotifen fumarate. For drug incubation, three CLs of each type were placed in 1 mL of 1 mg/mL drug-loading solution for 24 hours. The lenses were then extracted in 2 mL methanol for 2 hours. This process was repeated to obtain a total of three extraction cycles. Detection of natamycin, moxifloxacin, ketotifen fumarate, and timolol maleate were measured by absorbance at 305, 287, 297, and 295 nm, respectively. Results: The majority of the drugs were extracted after the first extraction cycle (P 0.05). Conclusions: This study provides a simple approach to determine drug uptake into CLs. This method can also be modified, such as changing the extraction time, extraction cycles, or extraction solvent to better suit other drugs and CL combinations. Translational Relevance: There is considerable interest in using CLs for ocular drug delivery. Accurately quantifying drug uptake on CLs has been a challenge. Hence, this study provides a simple method to quantify drug uptake in CLs. © 2018 The Authors.

DOI10.1167/tvst.7.2.11