Sub-micrometer axial resolution OCT for in-vivo imaging of the cellular structure of healthy and keratoconic human corneas

TitleSub-micrometer axial resolution OCT for in-vivo imaging of the cellular structure of healthy and keratoconic human corneas
Publication TypeJournal Article
Year of Publication2017
AuthorsBizheva, K., B. Tan, B. Maclelan, O. Kralj, M. Hajialamdari, D. Hileeto, and L. Sorbara
JournalBiomedical Optics Express
Volume8
Pagination800-812
Keywordsadult, Article, Biological tissues, Bowman membrane, Cellular structure, controlled study, cornea epithelium, cornea stroma, Corneal epithelium, Corneal pathologies, Diagnosis, female, human, human tissue, Image processing, imaging, Imaging systems, keratoconus, male, Ophthalmic optics, Ophthalmic optics and devices, Optical coherence tomography, Optical design, optical instrumentation, optical resolution, Optical tomography, Progressive damage, sensitivity and specificity, Spatial resolution, Subcellular structure, Tissue, Visual acuity
Abstract

Corneal degenerative conditions such as keratoconus (KC) cause progressive damage to the anterior corneal tissue and eventually severely compromise visual acuity. The ability to visualize corneal tissue damage in-vivo at cellular or sub-cellular level at different stages of development of KC and other corneal diseases, can aid the early diagnostics as well as the development of more effective treatment approaches for various corneal pathologies, including keratoconus. Here, we present the optical design of an optical coherence tomography system that can achieve 0.95 µm axial resolution in biological tissue and provide test results for the system’s spatial resolution and sensitivity. Corneal images acquired in-vivo with this system from healthy and keratoconic human subjects reveal the cellular and subcellular structure of the corneal epithelium, as well as the normal and abnormal structure of the Bowman’s membrane and the anterior corneal stroma. © 2017 Optical Society of America.

DOI10.1364/BOE.8.000800