In Vitro Effect of Lysozyme on Albumin Deposition to Hydrogel Contact Lens Materials

TitleIn Vitro Effect of Lysozyme on Albumin Deposition to Hydrogel Contact Lens Materials
Publication TypeJournal Article
Year of Publication2017
AuthorsOmali, N., L. Subbaraman, M. Heynen, Z. Fadli, C. Coles-Brennan, and L. Jones
JournalOptometry and Vision Science
KeywordsAlbumin adsorption, albuminoid, Albumins, Anti-Infective Agents, antiinfective agent, Bacterial, Bacterial binding, bacterial eye infection, Calibration curves, Contact lenses, Deposition, Enzymes, Eye Infections, human, Humans, hydrogel, Hydrogel contact lens, Hydrogels, Hydrophilic, hydrophilic contact lens, lysozyme, Lysozyme deposition, Lysozyme solutions, microbiology, Muramidase, Phosphate buffered saline solutions, Protein concentrations, Radioactivity

SIGNIFICANCE: Albumin deposition on contact lenses could be detrimental to contact lens (CL) wear because this may increase the risk of bacterial binding and reduce comfort. Lysozyme deposition on selected lens materials would reduce albumin deposition on lenses. PURPOSE: This study aims to determine if lysozyme deposition on CLs could act as a barrier against subsequent albumin adsorption, using an in vitro model. METHODS: Six hydrogel CL materials (etafilcon A, polymacon, nelfilcon A, omafilcon A, ocufilcon B, and nesofilcon A) were evaluated. Four CLs of each type were soaked in lysozyme solution for 16 hours at 37°C. Lysozyme-coated lenses were then placed in vials with 1.5 mL of artificial tear solution containing 125I-labeled albumin for 16 hours at 37°C with shaking. Four uncoated lenses of each type were used as controls. Lenses soaked in radiolabeled albumin were rinsed in a phosphate-buffered saline solution, and radioactive counts were measured directly on lenses using a gamma counter. Albumin uptake on lenses was measured using a calibration curve by plotting radioactive counts versus protein concentration. RESULTS: Results are reported as mean ± SD. Lysozyme-coated etafilcon A lenses exhibited lower levels of deposited albumin than uncoated etafilcon A lenses (58 ± 12 vs. 84 ± 5 ng/lens; P .05). Uncoated nesofilcon A lenses deposited the highest amount of albumin when compared with other uncoated lenses (P <.05). CONCLUSIONS: This study demonstrates that lysozyme deposited onto etafilcon A resists the deposition of albumin, which may potentially be beneficial to CL wearers. Copyright © 2017 American Academy of Optometry.