@article {21891,
	title = {Production of cellulosic butyrate and 3-hydroxybutyrate in engineered Escherichia coli},
	journal = {Applied Microbiology and Biotechnology},
	volume = {103},
	number = {13},
	year = {2019},
	note = {Miscevic, Dragan Srirangan, Kajan Kefale, Teshager Abedi, Daryoush Moo-Young, Murray Chou, C. Perry},
	pages = {5215-5230},
	abstract = {Being the most abundant renewable organic substance on Earth, lignocellulosic biomass has acted as an attractive and cost-effective feedstock for biobased production of value-added products. However, lignocellulosic biomass should be properly treated for its effective utilization during biotransformation. The current work aimed to demonstrate biobased production of butyrate and 3-hydroxybutyrate (3-HB) in engineered Escherichia coli using pretreated and detoxified aspen tree (Populus tremuloides) wood chips as the feedstock. Various bioprocessing and genetic/metabolic factors limiting the production of cellulosic butyrate and 3-HB were identified. With these developed bioprocessing strategies and strain engineering approaches, major carbons in the hydrolysate, including glucose, xylose, and even acetate, could be completely dissimilated during shake-flask cultivation with up to 1.68 g L-1 butyrate, 8.95 g L-1 3-HB, and minimal side metabolites (i.e., acetate and ethanol) being obtained. Our results highlight the importance of consolidating bioprocess and genetic engineering strategies for effective biobased production from lignocellulosic biomass.},
	author = {Miscevic, D. and Srirangan, K. and Abedi, D. and Moo-Young, M. and Chou, C. P.}
}
