<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Miscevic, D.</style></author><author><style face="normal" font="default" size="100%">Srirangan, K.</style></author><author><style face="normal" font="default" size="100%">Abedi, D.</style></author><author><style face="normal" font="default" size="100%">Moo-Young, M.</style></author><author><style face="normal" font="default" size="100%">Chou, C. P.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Production of cellulosic butyrate and 3-hydroxybutyrate in engineered Escherichia coli</style></title><secondary-title><style face="normal" font="default" size="100%">Applied Microbiology and Biotechnology</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2019</style></year></dates><number><style face="normal" font="default" size="100%">13</style></number><volume><style face="normal" font="default" size="100%">103</style></volume><pages><style face="normal" font="default" size="100%">5215-5230</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Being the most abundant renewable organic substance on Earth, lignocellulosic biomass has acted as an attractive and cost-effective feedstock for biobased production of value-added products. However, lignocellulosic biomass should be properly treated for its effective utilization during biotransformation. The current work aimed to demonstrate biobased production of butyrate and 3-hydroxybutyrate (3-HB) in engineered Escherichia coli using pretreated and detoxified aspen tree (Populus tremuloides) wood chips as the feedstock. Various bioprocessing and genetic/metabolic factors limiting the production of cellulosic butyrate and 3-HB were identified. With these developed bioprocessing strategies and strain engineering approaches, major carbons in the hydrolysate, including glucose, xylose, and even acetate, could be completely dissimilated during shake-flask cultivation with up to 1.68 g L-1 butyrate, 8.95 g L-1 3-HB, and minimal side metabolites (i.e., acetate and ethanol) being obtained. Our results highlight the importance of consolidating bioprocess and genetic engineering strategies for effective biobased production from lignocellulosic biomass.</style></abstract><notes><style face="normal" font="default" size="100%">Miscevic, Dragan Srirangan, Kajan Kefale, Teshager Abedi, Daryoush Moo-Young, Murray Chou, C. Perry</style></notes></record></records></xml>