<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">D. Miscevic</style></author><author><style face="normal" font="default" size="100%">J.-Y. Mao</style></author><author><style face="normal" font="default" size="100%">M. Moo-Young</style></author><author><style face="normal" font="default" size="100%">C. P. Chou</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">High-level heterologous production of propionate in engineered Escherichia coli</style></title><secondary-title><style face="normal" font="default" size="100%">Biotechnology and Bioengineering</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2020</style></year></dates><volume><style face="normal" font="default" size="100%">117</style></volume><pages><style face="normal" font="default" size="100%">1304-1315 </style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;span lang=&quot;EN-CA&quot;&gt;&lt;span style=&quot;line-height:107%&quot;&gt;&lt;span style=&quot;NewRoman&amp;quot;,serif&quot;&gt;Previously, we derived a propanologenic (i.e., 1-propanol-producing) bacterium &lt;i&gt;Escherichia coli&lt;/i&gt; strain by activating the &lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;span&gt;&lt;span style=&quot;line-height:107%&quot;&gt;&lt;span style=&quot;NewRoman&amp;quot;,serif&quot;&gt;genomic Sleeping beauty mutase (Sbm) operon&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;span lang=&quot;EN-CA&quot;&gt;&lt;span style=&quot;line-height:107%&quot;&gt;&lt;span style=&quot;NewRoman&amp;quot;,serif&quot;&gt;. The activated&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;span&gt;&lt;span style=&quot;line-height:107%&quot;&gt;&lt;span style=&quot;NewRoman&amp;quot;,serif&quot;&gt; Sbm pathway branches out of the &lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;span lang=&quot;EN-CA&quot;&gt;&lt;span style=&quot;line-height:107%&quot;&gt;&lt;span style=&quot;NewRoman&amp;quot;,serif&quot;&gt;tricarboxylic acid (&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;span&gt;&lt;span style=&quot;line-height:107%&quot;&gt;&lt;span style=&quot;NewRoman&amp;quot;,serif&quot;&gt;TCA) cycle at the succinyl-CoA node to form propionyl-CoA and its derived metabolites of 1-propanol and propionate.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;span&gt;&lt;span style=&quot;line-height:107%&quot;&gt;&lt;span style=&quot;NewRoman&amp;quot;,serif&quot;&gt; In this study, w&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;span&gt;&lt;span style=&quot;line-height:107%&quot;&gt;&lt;span style=&quot;NewRoman&amp;quot;,serif&quot;&gt;e targeted several TCA cycle genes encoding enzymes near the succinyl-CoA node for genetic manipulation in order to identify the individual contribution of the carbon flux into the Sbm pathway from the three TCA metabolic routes, i.e., oxidative TCA cycle, reductive TCA branch, and glyoxylate shunt.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;span&gt;&lt;span style=&quot;line-height:107%&quot;&gt;&lt;span style=&quot;NewRoman&amp;quot;,serif&quot;&gt; For the control strain &lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;span&gt;&lt;span style=&quot;line-height:107%&quot;&gt;&lt;span style=&quot;NewRoman&amp;quot;,serif&quot;&gt;CPC-Sbm in which propionate biosynthesis occurred under relatively anaerobic conditions, the carbon flux into the Sbm pathway were primarily derived from the reductive TCA branch; and &lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;span lang=&quot;EN-CA&quot;&gt;&lt;span style=&quot;line-height:107%&quot;&gt;&lt;span style=&quot;NewRoman&amp;quot;,serif&quot;&gt;both succinate availability and the SucCD-mediated interconversion of succinate/succinyl-CoA were critical for such carbon flux redirection. While the oxidative TCA cycle normally had a minimal contribution to the carbon flux redirection, the glyoxylate shunt could be an alternative and effective carbon flux contributor under aerobic conditions.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;span lang=&quot;EN-CA&quot;&gt;&lt;span style=&quot;line-height:107%&quot;&gt;&lt;span style=&quot;NewRoman&amp;quot;,serif&quot;&gt; With mechanistic understanding of such &lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;span&gt;&lt;span style=&quot;line-height:107%&quot;&gt;&lt;span style=&quot;NewRoman&amp;quot;,serif&quot;&gt;carbon flux redirection, metabolic strategies based on blocking the oxidative TCA cycle (via ∆&lt;i&gt;sdhA&lt;/i&gt; mutation) and deregulating the glyoxylate shunt (via ∆&lt;i&gt;iclR&lt;/i&gt; mutation) were developed to enhance the carbon flux redirection and, therefore, propionate biosynthesis, achieving a high propionate titer of 30.9 g l&lt;sup&gt;-1&lt;/sup&gt; with an overall propionate yield of 49.7% upon fed-batch cultivation of the double mutant strain CPC-Sbm∆&lt;i&gt;sdhA&lt;/i&gt;∆&lt;i&gt;iclR&lt;/i&gt; under aerobic conditions. The results also suggest that the Sbm pathway could be metabolically active under both aerobic and anaerobic conditions.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;</style></abstract></record></records></xml>