@article{7,
  keywords = {2D Nanoparticle Synthesis for Devices and Sensors},
  author = {Liena Zaidan and Inna Novodchuk and Alexandru Nica and Alexander Xu and Christopher Lloyd and Reza Karimi and Joseph Sanderson and Michal Bajcsy and Mustafa Yavuz},
  title = {Rapid, Selective, and Ultra-Sensitive Electronic Field Effect Detection of Escherichia coli},
  abstract = {<p>We report the detection of live Escherichia coli (E. coli) in liquid samples using a biosensor based on a field effect transistor (FET) biosensor with B/N co-coped graphene oxide (GO) gel (BN-GO) as the transducer material. The FET was functionalized with antibodies to detect E. coli K12 O-antigens in phosphate-buffered saline (PBS). The biosensor detected the presence of planktonic E. coli bacterial cells within a mere 2 minutes. The biosensor exhibited a limit of detection (LOD) of 10 cells per sample, which can be extrapolated to a limit of detection at the level of a single cell per sample and a detection range of at least 10–108 CFU/mL. The selectivity of the biosensor for E. coli was demonstrated using Bacillus thuringiensis (B. thuringiensis) as a sample contaminant. We also present a comparison of our functionalized BN-GO FET biosensor with established detection methods of E. coli k12 bacteria, as well as with state-of-the-art detection mechanisms.</p>
},
  year = {2024},
  journal = {preprints.org},
  month = {2024/6/24},
  url = {https://www.preprints.org/manuscript/202406.1630/v1},
  doi = {10.20944/preprints202406.1630.v1},
}