|Title||Evaluation of hypoxic swelling of human cornea with high speed ultrahigh resolution optical coherence tomography|
|Publication Type||Conference Paper|
|Year of Publication||2009|
|Authors||Bizheva, K., C. Hyun, J. A. Eichel, S. Hariri, D. A. Clausi, P. Fieguth, T. Simpson, and N. Hutchings|
|Conference Name||SPIE BIOS (Biomedical Optics)|
|Conference Location||San Jose, CA, USA|
Hypoxia induced corneal swelling was observed and evaluated in healthy human volunteers by use of high speed, ultrahigh resolution optical coherence tomography (UHROCT). Two dimensional corneal images were acquired at a speed of 47,000 A-scans/s with 3m x 10m (axial x lateral) resolution in corneal tissue. The UHROCT tomograms showed clear visualization of all corneal layers, including the Bowman's layer and the Descemet's membrane - Endothelium complex. A segmentation algorithm was developed and used for automatic detection of the boundaries of the different corneal layers and evaluation the individual layer thickness as a function of location. Corneal hypoxia was induced by wear of a soft contact lens (SCL) and an eye patch by 2 healthy volunteers for duration of 3 hours. The thickness of all corneal layers was measured as a function of time, prior to, with and after removal of the SCL. Results from the hypoxia study showed different rates of swelling and de-swelling of the individual corneal layers. About 10% increase in the total cornea thickness was observed, similar to the changes in the stroma, the Bowman's membrane swelled by 20%, while no significant change in the thickness was observed in the Descemet's - Endothelium complex.