Jüttner | Canadian Phycological Culture Centre

Reference: Jüttner et al., 1983.

This medium is used successfully for some cyanobacteria. Vitamins may be added. The CPCC currently adds f/2 vitamins to the Jüttner medium for all strains.

Stock	Substance	Concentration	Amount added to medium (mL/L)
1.	CaCl₂•2H₂O	8.8 g/L	10 mL
1.	NaNO₃	68 g/L	10 mL
2.	K₂HPO₄*	7.0 g/L*	10 mL
3.	MgSO₄•7H₂O	9.9 g/L	10 mL
4.	NaFeEDTA	367 mg/L	10 mL
5.	Trace metal solution	See below	10 mL
6.	f/2 vitamin solution	See below	1 mL

*May also prepare a 9.1 g/L stock solution of K₂HPO₄•3H₂O instead of 7.0 g/L of K₂HPO₄

Adjust the pH of the medium to approximately 9 prior to autoclaving. However, please note that the CPCC has been pH adjusting the medium to approximately 8 (i.e., 7.5-8.5). Store stock solutions 1-5 in the fridge in the dark. It is especially important to keep Stock 4 in the dark, so use amber bottle and/or cover the bottle in tin foil. The f/2 vitamins (Stock 6) should be stored in the freezer in 1 mL aliquots and passively thawed as needed.

Trace metal solution

Add 100 mL Trace Elements Solution I plus 100 mL Trace Elements Solution II to 800 mL purified H₂O.

Trace elements solution I:

Substance	Concentration (mg/L)
1. H₃BO₃	618 mg
2. MnCl₂•4H₂O	1979 mg
3. ZnSO₄•7H₂O	58 mg
4. CuSO₄•5H₂O	50 mg
5. CoSO₄•7H₂O	56 mg

Trace elements solution II:

Substance	Concentration (mg/L)
1. Na₂MoO₄•2H₂O	484 mg

Add each substance in the order that they appear here and ensure that each is visually dissolved prior to adding the next on the list. After all substances have been added the stock solution should be topped up with purified water to 1000 mL. Then combine 100 mL of Trace Elements Solution I with 100 mL of Trace Elements Solution II and top up to 1000 mL.

f/2 vitamin solution

Substock solutions:

First prepare separate substock solutions of cyanocobalamin (Vitamin B12) and biotin in purified water (e.g., Milli-Q, Nanopure, distilled water).

Substock	Substance	Concentration
1.	Vitamin B12 (Cyanocobalamin)	10 mg/10 mL
2.	Biotin	5 mg/50 mL

Working stock solution:

Then prepare the final working solution by combining the following amounts of the two substock solutions plus thiamine HCl to 100 mL of purified water.

Substance	Concentration (per 100 mL)
1. Vitamin B12 (Cyanocobalamin)	0.1 mL
2. Biotin	1.0 mL
3. Thiamine HCl	20 mg

Dispense the working solution as 1 mL aliquots in cryovials for convenient use when preparing the medium. Store the remainder of the primary stocks and working solution in polyethylene bottles. Wrap vials/bottles with Parafilm to avoid moisture loss and store all solutions frozen.

References: Guillard and Ryther 1962, Guillard 1975