In this instrument, the collision cell of a conventional QTof has been replaced with 2 collision cells (Trap and Transfer) and between these is the Travelling Wave Ion Mobility (TWIM) cell. CID can be carried out in the Trap and/or Transfer with or without TWIM.
The Synapt is capable of performing mass spectrometry (MS), tandem mass spectrometry (MS/MS) as well as high resolution/accurate mass and is interfaced to a Waters I-class UPLC for LC/MS(MS) and an Agilent 7890B GC for AP GC/MS(MS). In addition, this system has integrated TWIM, a new orthogonal dimension of ion separation*.
*Ions are separated based on their average collisional cross section (size, shape and charge state) not their m/z ratio. This capability can be seamlessly incorporated into any analytical workflow (so-called HD modes) ie LC/MS(MS) to further deconvolute complex mixtures or separate isomeric species.
Ionization sources:
- Combined Electrospray ionization (ESI) and Atmospheric Pressure Chemical Ionization (APCI) source (ESCI) optimised for analytical LC/MS(MS) flow rates (>100uL/min to 1mL/min).
- APCI with GC to allow analysis of compounds not amenable to LC/MS(MS) analysis.
Analysis is possible for a wide range of (bio)chemical species:
- Molecular mass determination: Time-of-flight mass spectrometry (TOF/MS) analysis
- High resolution, exact mass determination: TOF/MS analysis with lock mass yielding ~1ppm precision with lock mass correction
- MS/MS analysis for structure elucidation using set or ramped collision energy (CE)
- Data Independent and Data Dependant analysis (DIA and DDA)
- Analytical UPLC/MS and/or UPLC/MS/MS may be performed by special arrangement with the Facility. Similarly for GC/MS(MS).
- Travelling Wave Ion Mobility which can be combined with batch introduction/infusion, UPLC/MS(MS) and GC/MS(MS).
- Ultra High-performance liquid chromatography (UPLC) and gas chromatographic (GC) conditions must have been optimised previously and a suitable column must be provided for these analyses.
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