High throughput fatty acid analyses
Gas chromatography with flame ionization detection using Varian 3900 or Agilent 7890A instruments.
Lipidomics
Ultra high-performance liquid chromatography with tandem mass spectrometry using a Thermo Scientific Q-Exactive Orbitrap or a Waters Synapt G2Si HDMS hybrid quadrupole/time-of-flight mass spectrometer with integrated travelling wave ion mobility.
Gene expression
RNA extraction and RT-PCR for mRNA expression and immunoblotting for protein quantification.