Research publications

SR. PuMMP Lab publications

Please see the below publications from the SR. PuMMP lab. If you would like any further information, feel free to email Professor Russell Tupling.

Research themes:

• Sarcolipin and obesity

• SERCA and hsp70 interaction

• Skeletal muscle HSP expression and response to exercise

• SR Ca²⁺ handling, muscle contractility, fatigue and disease

Sarcolipin and obesity

• Gamu D, Bombardier E, Smith IC, Fajardo VA, Tupling AR. Sarcolipin provides a novel muscle-based mechanism for for adaptive thermogenesis. Exerc Sport Sci Rev. 2014 Jul;42(3):136-42. 
• Smith IC, Bombardier E, Vigna C, Tupling AR. ATP consumption by sarcoplasmic reticulum Ca2+ pumps accounts for 40-50% of resting metabolic rate in mouse fast and slow twitch skeletal muscle. PLoS ONE 8(7): e68924, 2013.
• Bombardier E, Smith IC, Gamu D, Fajardo VA, Vigna C, Sayer RA, Gupta SC,  Bal NC, Periasamy M, Tupling AR. Sarcolipin trumps β-Adrenergic receptor signaling as the favored mechanism for muscle-based diet-induced thermogenesis. FASEB J. 27(9): 3871-3878, 2013.
• Bombardier E, Smith IC, Vigna C, Fajardo VA, Tupling AR. Ablation of sarcolipin decreases the energy requirements for Ca2+ transport by sarco(endo)plasmic reticulum Ca2+-ATPases in resting skeletal muscle. FEBS Lett. 587(11): 1687-1692, 2013.
• Bal NC, Maurya SK, Sopariwala DH, Sahoo SK, Gupta SC, Shaikh SA, Pant M, Rowland LA, *Bombardier E, Goonasekera SA, Tupling AR, Molkentin JD, Periasamy M. Sarcolipin is a newly identified regulator of muscle-based thermogenesis in mammals. Nat. Med. 18(10): 1575-9, 2012.
• Tupling AR, Bombardier E, Gupta SC, Hussain D, Vigna C, Bloemberg D, Quadrilatero J, Trivieri MG, Babu GJ, Backx PH, Periasamy M, Maclennan DH, Gramolini AO. Enhanced Ca2+ transport and muscle relaxation in skeletal muscle from sarcolipin-null mice. Am. J. Physiol. Cell Physiol. 301(4): C841-9, 2011.
• MacLennan DH, Asahi M, Tupling AR. The regulation of SERCA type pumps by phospholamban and sarcolipin. Ann. N.Y. Acad. Sci. 986: 472–80, 2003.
• Tupling AR, Asahi M, MacLennan DH. Sarcolipin over-expression in rat slow-twitch muscle inhibits sarcoplasmic reticulum Ca2+ uptake and impairs contractile function. J. Biol. Chem. 277(47): 44740–6, 2002.

SERCA and hsp70 interaction

• Fu M, Tupling AR. Protective effects of Hsp70 on the structure and function of SERCA2a expressed in HEK-293 cells during heat stress. Am. J. Physiol. Heart Circ. Physiol. 296: H1175-83, 2009.
• Tupling AR. Protection of heart and skeletal muscle by heat shock proteins. Appl. Physiol. Nutr. Metab. 33(5): 1021-22, 2008.
• Tupling AR, *Bombardier E, Vigna C, Quadrilatero J, Fu M. Interaction between Hsp70 and the SR Ca²⁺ pump: A potential mechanism for cytoprotection in heart and skeletal muscle. Appl. Physiol. Nutr. Metab. 33(5): 1023-32, 2008.
• Tupling AR, Gramolini AO, Duhamel TA, Kondo H, Asahi M, Tsuchiya SC, Borrelli MJ, Lepock JR, Otsu K, Hori M, MacLennan DH, Green HJ. HSP70 binds to the fast-twitch skeletal muscle Sarco (endo)plasmic reticulum Ca2+-ATPase (SERCA1a) and prevents thermal inactivation. J. Biol. Chem. 279(50): 52382 –9, 2004.
• Tupling AR, Green HJ, Roy BD, Grant S, Ouyang J. Paradoxical effects of prior activity on human sarcoplasmic reticulum Ca2+-ATPase response to exercise. J. Appl. Physiol. 95(1): 138–44, 2003.

Skeletal muscle HSP expression and response to exercise

• Bombardier E, Vigna C, Bloemberg D, Quadrilatero J, Tiidus PM, Tupling AR. The role of estrogen receptor-α in estrogen-mediated regulation of basal and exercise-induced Hsp70 and Hsp27 expression in rat soleus. Can. J. Physiol. Pharmacol. 91(10): 823-829, 2013.
• Bombardier E, Vigna C, Iqbal S, Tiidus PM, Tupling AR. Effects of ovarian sex hormones and downhill running on fiber type-specific Hsp70 expression in rat soleus. J. Appl. Physiol. 106(6): 2009-15, 2009.
• Tupling AR, Bombardier E, Stewart RD, Vigna C, Aqui AE. Muscle fiber type specific response of Hsp70 expression in human quadriceps following acute isometric exercise. J. Appl. Physiol. 103: 2105-11, 2007.

SR Ca²⁺ handling, muscle contractility, fatigue and disease

• Smith IC, Gittings W, Huang J, McMillan EM, Quadrilatero J, Tupling AR and Vandenboom R. Potentiation in mouse lumbrical muscle without myosin light chain phosphorylation: Is resting calcium responsible? J. Gen. Physiol. 141(3): 297-308, 2013.
• Smith IC, Huang J, Quadrilatero J, Tupling AR, Vandenboom R. Posttetanic potentiation in mdx muscle. J. Muscle Res. Cell Motil. 31(4): 267-77, 2010.
• Middlekauff HR, Vigna C, Verity MA, Fonarow GC, Horwich TB, Hamilton MA, Shieh P, Tupling AR. Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism? J. Card. Fail. 18(9): 724-33, 2012.
• Zvaritch E, Kraeva N, Bombardier E, McCloy RA, Depreux F, Holmyard D, Kraev A, Seidman CE, Seidman JG, Tupling AR, MacLennan DH. Ca²⁺dysregulation in Ryr1^I4895T/wt mice causes a congenital myopathy with progressive formation of minicores, cores and nemaline rods. Proc Natl Acad Sci U S A. 106(51): 21813-8, 2009.
• Tupling AR. The decay phase of Ca²⁺ transients in skeletal muscle: Regulation and physiology. Appl. Physiol. Nutr. Metab. 34(3): 373-6, 2009.
• Tupling AR, Vigna C, Ford RJ, Tsuchiya SC, Graham DA, Denniss SG, Rush JW. Effects of Buthionine Sulfoximine (BSO) Treatment on Diaphragm Contractility and SR Ca2+ Pump Function in Rats. J. Appl. Physiol. 103: 1921-28, 2007.
• Tupling AR. The sarcoplasmic reticulum in fatigue and disease. Can. J. Appl. Physiol. 29(3): 308-29, 2004.
• Pan Y, Zvaritch E, Tupling AR, Rice WJ, de Leon S, Rudnicki M, McKerlie C, Banwell B, MacLennan DH. Targeted disruption of the ATP2A1 gene encoding the sarco(endo)plasmic reticulum Ca2+-ATPase isoform 1 (SERCA1) impairs diaphragm function and is lethal in neonatal mice. J. Biol. Chem. 278: 13367 –75, 2003.