Stephens, R.F., Mourad, O., Mehta, A., Fux, J. and Spafford, J.D. (2018) "Analyses of the unique cysteine-enriched extracellular turrets above Cav3 channels" (submitted)
Fux, J.E., Mehta, A., Moffatt, J. and Spafford, J.D. (2018) "Eukaryotic Voltage-Gated Sodium Channels: On Their Origins, Asymmetries, Losses, Diversification and Adaptations". Frontiers in Physiology. Membrane Physiology and Membrane Biophysics. https://doi.org/10.3389/fphys.2018.01406
Chemin, J., Taiakina, V., Monteil, A., Piazza, M., Guan, W., Stephens, R.F., Kitmitto, A., Pang, Z.P., Dolphin, A.C., Perez-Reyes, E., Dieckmann, T., Guillemette, J.G., Spafford, J.D. (2017) “Ca regulates Cav3 T-type channels at their gating brake”. Journal of Biological Chemistry. 292(49) 20010-20031. doi: 10.1074/jbc.M117.807925
Stephens, R.F., Guan, W., Zhorov, B.S. and Spafford, J.D. (2015) Selectivity filters and cysteine-rich extracellular loops in voltage-gated sodium, calcium, and NALCN channels. Frontiers in Physiology. Membrane Physiology and Membrane Biophysics. http://dx.doi.org/10.3389/fphys.2015.00153
Guan, W., Stephens, R.F. and Spafford, J.D. (2014) "Cav3 T-type channels as drug targets for treating epilepsy" Future Neurology. 9(6)563-566. DOI 10.2217/fnl.14.42
Boone, A.N., Senatore, A., Chemin, J., Monteil, A., Spafford, J.D. (2014) "Gd3+ and calcium sensitive, sodium leak currents are features of weak membrane-glass seals in patch clamp recordings” PLoS ONE 9(6): e98808. doi:10.1371/journal.pone.0098808
Senatore, A., Guan, W., Boone, A.N., Spafford, J.D. (2014) "T-type calcium channels become highly sodium-permeable using an alternate extracellular turret region (S5-P) outside the selectivity filter” Journal of Biological Chemistry. 289(17) 11952‐11969. doi: 10.1074/ jbc.M114.551473.
Dawson, T.F., Boone, A.N., Senatore, A., Piticaru, J., Thiyagalingam, S., Jackson, D., Davison, A., Spafford, J.D. (2014) "Gene splicing of an invertebrate beta subunit (LCavβ) in the N-terminal and HOOK domains and its regulation of LCav1 and LCav2 calcium channels" PLoS ONE 9(4): e92941. doi:10.1371/ journal.pone.0092941
Senatore, A., Spafford, J.D. (2014) “Chapter 1 - Physiology and pathology of voltage-gated T-Type calcium channels”, in T-type calcium channels in basic and clinical sciences. Springer. Schaffer, Stephen W.; Wu, Songwei; Li, Ming (Eds.), pp. 3-18.
Senatore, A., Guan, W., Spafford, J.D. (2014) "Cav3 T-type channels: Regulators for gating, membrane expression and cation selectivity” Special issue "T-type (Cav3) calcium channels in health and disease" for Pfluger Archives: European Journal of Physiology. 466 (4), 645-660.
Taiakina, V., Boone, A.N., Fux, J., Senatore, A., Weber-Adrian, D., Guillemette, J.G., Spafford, J.D. (2013) "The calmodulin-binding, short linear motif, NSCATE is conserved in L-type channel ancestors of vertebrate Cav1.2 and Cav1.3 channels” PLoS ONE 8(4):e61765. doi: 10.1371/journal.pone.0061765.
Senatore, A. and Spafford, J.D. (2013) “A uniquely adaptable pore is consistent with NALCN being an ion sensor” Channels. 2013 Feb 26;7(2).
Senatore, A., Monteil, A., van Minnen, J., Smit, A.B., Spafford, J.D. (2013) “NALCN leak conductance channel expresses in vitro and in situ with alternative selectivity filters resembling calcium channels or sodium channels” PLoS ONE 8(1): e55088. doi:10.1371/ journal.pone. 0055088.
Senatore, A., Zhorov B S., Spafford J. D.. (2012) Cav3 T‐type calcium channels. WIREs Membrane Transport and Signals, 1: 467-491. doi: 10.1002/wmts.41
Senatore, A., Spafford, J.D. (2012) Gene transcription and splicing of T-type channels are evolutionarily-conserved strategies for regulating channel expression and gating. PLoS ONE 7(6): e37409. doi:10.1371/ journal.pone.0037409
Senatore, A., Boone, A.N., Lam, S, Dawson, T. F, Zhorov, B.S., Spafford, J.D. (2011) “Mapping of dihydropyridine binding residues in a less sensitive invertebrate L-type calcium channel (LCav1)” Channels (5(2):173-87.
Senatore, A., Boone, A., Spafford, J.D. (2011) “Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells” Journal of Visualized Experiments (JoVE). Jan 19;(47). pii: 2314. doi: 10.3791/2314.
Huang, X., Senatore, A., Dawson, T.F. Quan, Q., Spafford, J.D. (2010) G proteins modulate invertebrate synaptic calcium channel (LCav2) differently from the classical voltage-dependent regulation of mammalian Cav2.1 and Cav2.2 channels Journal of Experimental Biology. 213(Pt 12):2094-103.
Senatore, A., Spafford, J.D. (2010) Transient and big are key features of an invertebrate T-type channel (LCav3) from the central nervous system of Lymnaea stagnalis Journal of Biological Chemistry. 285, 7447-7458.
Zhang, Y., Helm, J., Senatore, A., Spafford, J.D., Kaczmarek, L. and Jonas E. (2008) PKC-induced intracellular trafficking of CaV2.1 precedes its rapid recruitment to the plasma membrane. Journal of Neuroscience. 28(10):2601-12.
Flynn,R., Hameed, S., Chen, L., Spafford, J.D., and Zamponi, G.W. (2008) Molecular determinants of Rem2 regulation of N-type calcium channels. Biochemical and Biophysical Research Communications. 368(3):827-31.
Pham P., Senatore, A. and Spafford, J.D. (2007) Ion channels: from development to disease. Encyclopedia of Neuroscience. Binder, M.D.; Hirokawa, N.; Windhorst, U.; Hirsch, M.C. (Eds.). Springer Verlag GmbH, Heidelberg.
Senatore, A. and Spafford, J.D. (2007) Calcium channels: Regulation of gene expression. Encyclopedia of Neuroscience. Binder, M.D.; Hirokawa, N.; Windhorst, U.; Hirsch, M.C. (Eds.). Springer Verlag GmbH, Heidelberg.
Spafford, J.D., Dunn, T., P., Smit, A.B., Syed, N.I. and Zamponi, G.W. (2006) In vitro characterization of L-type calcium channels and their contribution to firing behavior in invertebrate respiratory neurons. Journal of Neurophysiology. 95(1):42-52.
Nierop, P., Bertrand, S., Munno, D., Gouwenberg, Y., van Minnen, J., Spafford, J.D., Syed, N.I., Bertrand, D. and Smit, A.B. (2006) Identification and functional expression of a family of nicotinic acetylcholine receptor subunits in the central nervous system of the mollusc Lymnaea stagnalis. Journal of Biological Chemistry. 281(3):1680-1691.
Spafford, J.D., van Minnen, J., Larsen, P., Smit, A.B., Syed, N.I. and G.W. Zamponi (2004) Uncoupling of calcium channel alpha1 and beta subunits in developing neurons. Journal of Biological Chemistry. 279:41157-41167.
Spafford, J.D. and Zamponi, G.W. (2003) Functional interactions of presynaptic calcium channels with the neurotransmitter release machinery. Current Opinion in Neurobiology 13:1-7.
Szule, J.A., Jarvis, S.E., Hibbert, J.E, Spafford, J.D., Braun, J.E., Zamponi, G.W., Wessel, G.M., and Coorssen, J.R. (2003) Calcium-triggered membrane fusion proceeds independently of specific presynaptic proteins. Journal of Biological Chemistry. 278:24251-24254.
Spafford, J.D., Chen, L., Feng, Z.P. Smit, A.B. and Zamponi, G.W. (2003) Expression and modulation of an invertebrate presynaptic calcium channel a1 subunit homolog. Journal of Biological Chemistry. 278:21178-21187.
Feng, Z.P., Doering, C.J., Winkfein, R, Beedle, A.M., Spafford, J.D., and Zamponi, G.W. (2003) Determinants of w-conotoxin MVIIA (ziconotide) block of transiently expressed HVA and LVA calcium channels. Journal of Biological Chemistry. 278: 20171-20178.
Spafford, J.D., Munno, D.W., Nierop, P., Feng, Z-P., Jarvis, S.E., Gallin, W.J. Smit, A.B., Zamponi, G.W., Syed, N.I. (2003) Calcium channel structural determinants of invertebrate synaptic transmission. Journal of Biological Chemistry. 278:4258-4267.
Hemmings S.J. and Spafford, J.D. (2000) Neonatal STZ model of type II diabetes mellitus in the Fischer 344 rat: characteristics and assessment of the status of the hepatic adrenergic receptors. International Journal of Biochemistry and Cell Biology. 32(8):905-19.
Spafford, J.D., Spencer, A.N. and Gallin, W.J. (1999) Genomic organization of a voltage-gated Na+ channel (PpSCN1) in a primitive metazoan: Evolutionary implications. Receptors and Channels. 6:493-506.
Grigoriev, N.G., Spafford, J.D. and Spencer, A.N. (1999) Modulation of jellyfish Shaker K+ channels by external potassium ions. Journal of Neurophysiology. 82(4):1728-1739.
Grigoriev, N.G., Spafford, J.D. and Spencer, A.N. (1999) Residues in the jellyfish Shaker-like channel (jShak2) involved in modulation by external potassium. Journal of Neurophysiology. 82(4):1740-1747.
Grigoriev, N.G., Spafford, J.D. and Spencer, A.N. (1999) Positive feed-back of potassium currents by effluxing potassium: effects of levels of expression of a jellyfish Shaker channel (jShak2) in Xenopus oocytes. Journal of Physiology. 517:25-33.
Spafford, J.D., Spencer, A.N. and Gallin, W.J. (1998) A putative voltage-gated sodium channel alpha subunit (PpSCN1) from the hydrozoan jellyfish, Polyorchis penicillatus: structural comparisons and evolutionary considerations. Biochemical and Biophysical Research Communications. 244(3): 772-780.
Grigoriev, N.G., Spafford, J.D., Gallin, W.J. and Spencer, A.N. (1997) Voltage sensing in jellyfish Shaker potassium channels. Journal of Experimental Biology. 200(22): 2919-2926.
Grigoriev, N.G., Spafford, J.D., Przysiezniak, J.P. and Spencer, A.N. (1996) A cardiac-like sodium current in motor neurons of jellyfish. Journal of Neurophysiology. 76(4): 2240-2249.
Spafford, J.D., Grigoriev, N.G. and Spencer, A.N. (1996) Pharmacological properties of voltage-gated Na+ currents in motor neurons from a hydrozoan jellyfish Polyorchis penicillatus. Journal of Experimental Biology. 199:941-948.