Publications
Heterologous expression of lipase in Escherichia coli is limited by folding and disulfide bond formation. Applied Microbiology and Biotechnology, 81, 79-87.
. (2008). Effect of folding factors in rescuing unstable heterologous lipase B to enhance its overexpression in the periplasm of Escherichia coli. Applied Microbiology and Biotechnology, 79, 1035-1044.
. (2008). Characterization of the T7 promoter system for expressing penicillin acylase in Escherichia coli. Applied Microbiology and Biotechnology, 72, 529-536.
. (2006). Chaperone-mediated folding and maturation of the penicillin acylase precursor in the cytoplasm of Escherichia coli. Applied and Environmental Microbiology, 71, 6247-6253. 2005_chaperone-mediated_folding_and_maturation_of_penicillin_acylase_precursor_in_the_cytoplasm_of_escherichia_coli.pdf
(2005). Cytoplasmic overexpression, folding, and processing of penicillin acylase precursor in Escherichia coli. Biotechnology Progress, 21, 1357-1365.
. (2005). Disulfide bond formation and its impact on the biological activity and stability of recombinant therapeutic proteins produced by Escherichia coli expression system. Biotechnology Advances, 29, 923-929. 2011_disulfide_bond_formation.pdf
. (2011). Molecular manipulation associated with disulfide bond formation to enhance the stability of recombinant therapeutic protein. Protein Expression and Purification, 75, 28-39.
. (2011). Effect of aberrant disulfide bond formation on protein conformation and molecular property of recombinant therapeutics. Pure and Applied Chemistry, 82, 149-159.
. (2010). Structural identification of recombinant human CD83 mutant variant as a potent therapeutic protein. Protein Expression and Purification, 73, 140-146.
(2010). Developing an RNase-free bioprocess to produce pharmaceutical-grade plasmid DNA using selective precipitation and membrane chromatography. Separation and Purification Technology, 83, 121-129.
(2011). Potential application of hydrogel-based strong anion-exchange membrane for plasmid DNA purification. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 879, 564-572.
(2011).