Applied Virus and Complex Biologics Bioprocessing Research Lab
200 Univeristy Avenue West
Waterloo, Ontario, Canada
N2L 3G1
Contact Marc Aucoin, Director, Applied Virus and Complex Biologics Bioprocessing Research Lab
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Ansorge, S., O. Henry, M. Aucoin, R. Voyer, J. P. Carvell, and A. Kamen, "Monitoring the cell size distribution of mammalian cell cultures using on-line capacitance measurements", Cells and Culture: Springer, pp. 853–859, 2010.
Aucoin, M. G., D. Jacob, P. Singh Chahal, J. Meghrous, A. Bernier, and A. A. Kamen, "Virus-like particle and viral vector production using the baculovirus expression vector system/insect cell system", Baculovirus and Insect Cell Expression Protocols: Springer, pp. 281–296, 2007.
Aucoin, M. G., M. Perrier, and A. A. Kamen, "Increasing Adeno-Associated Virus Specific Yield in Insect Cell Culture by Increasing Temperature", The 2006 Annual Meeting, 2006.
Aucoin, M., Fluorescent influenza-like particles and control over their composition, 2016.
Aucoin, M. G., "Graphical analysis of flow cytometer data for characterizing controlled fluorescent protein display on phage (vol 81, pg 1031, 2012)", CYTOMETRY PART A, vol. 83, no. 6: WILEY-BLACKWELL 111 RIVER ST, HOBOKEN 07030-5774, NJ USA, pp. 592–592, 2013.
Aucoin, M. G., J. A. Mena, and A. A. Kamen, "Bioprocessing of baculovirus vectors: a review", Current gene therapy, vol. 10, no. 3: Bentham Science Publishers, pp. 174–186, 2010.
Aucoin, M. G., and M. Jolicoeur, "GRADUATE EDUCATION-Is There Room In the Graduate Curriculum To Learn How To Be a Grad Student? An Approach Using a Graduate-Level Biochemical Engineering Course", Chemical Engineering Education, vol. 43, no. 4, pp. 306, 2009.
Aucoin, M. G., and M. Jolicoeur, "Is there room in the graduate curriculum to learn how to be a grad student? An approach using a graduate-level biochemical engineering course", Chemical Engineering Education, vol. 43, no. 4, pp. 306–312, 2009.
Aucoin, M. G., M. Perrier, and A. A. Kamen, "Critical assessment of current adeno-associated viral vector production and quantification methods", Biotechnology advances, vol. 26, no. 1: Elsevier, pp. 73–88, 2008.
Aucoin, M. G., M. Perrier, and A. A. Kamen, "Improving AAV vector yield in insect cells by modulating the temperature after infection", Biotechnology and bioengineering, vol. 97, no. 6: Wiley Online Library, pp. 1501–1509, 2007.
Aucoin, MG., D. Jacob, PS. Chahal, J. Meghrous, A. Bernier, and AA. Kamen, "Virus-like Particle and Viral Vector Production Using the BEVS/Insect Cell System: Adeno-Associated Virus-Based Products", METHODS IN MOLECULAR BIOLOGY-CLIFTON THEN TOTOWA-, vol. 388: HUMANA PRESS, pp. 281, 2007.
Aucoin, M. G., V. McMurray-Beaulieu, F. Poulin, E. B. Boivin, J. Chen, F. M. Ardelean, M. Cloutier, Y. J. Choi, C. B. Miguez, and M. Jolicoeur, "Identifying conditions for inducible protein production in E. coli: combining a fed-batch and multiple induction approach", Microbial cell factories, vol. 5, no. 1: BioMed Central, pp. 1–13, 2006.
Aucoin, M. G., M. Perrier, and A. A. Kamen, "Production of adeno-associated viral vectors in insect cells using triple infection: Optimization of baculovirus concentration ratios", Biotechnology and bioengineering, vol. 95, no. 6: Wiley Online Library, pp. 1081–1092, 2006.
Aucoin, M. G., F. A. Erhardt, and R. L. Legge, "Hyperactivation of Rhizomucor miehei lipase by hydrophobic xerogels", Biotechnology and bioengineering, vol. 85, no. 6: Wiley Online Library, pp. 647–655, 2004.
Aucoin, M. G., and R. L. Legge, "Effects of supercritical CO2 exposure and depressurization on immobilized lipase activity", Biotechnology letters, vol. 23, no. 22: Springer, pp. 1863–1870, 2001.
Aucoin, M. Gordon, Characterization and optimization of the production of adeno-associated viral vectors using a baculovirus expression vector/insect cell system: ProQuest, 2007.
Aucoin, M. Gordon, Effect of Organically Modified Silica Xerogels on Rhizomucor Miehei Lipase Activity: University of Waterloo, 2002.
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Blondeel, E. J. M., and M. G. Aucoin, "Supplementing glycosylation: a review of applying nucleotide-sugar precursors to growth medium to affect therapeutic recombinant protein glycoform distributions", Biotechnology advances, vol. 36, no. 5: Elsevier, pp. 1505–1523, 2018.
Blondeel, E. J. M., R. Ho, S. Schulze, S. Sokolenko, S. R. Guillemette, I. Slivac, Y. Durocher, G. J Guillemette, B. J. McConkey, D. Chang, et al., "An omics approach to rational feed: enhancing growth in CHO cultures with NMR metabolomics and 2D-DIGE proteomics", Journal of biotechnology, vol. 234: Elsevier, pp. 127–138, 2016.
Blondeel, E. J. M., K. Braasch, T. McGill, D. Chang, C. Engel, M. Spearman, M. Butler, and M. G. Aucoin, "Tuning a MAb glycan profile in cell culture: Supplementing N-acetylglucosamine to favour G0 glycans without compromising productivity and cell growth", Journal of biotechnology, vol. 214: Elsevier, pp. 105–112, 2015.
Boegel, S. J., M. A. Gabriel, M.R. Sasges, B. Petri, J. C. Ang, M. D'Agostina, A. Zhang, M. Miller, S. M. Meunier, and M. Gordon Aucoin, "Ultraviolet Disinfection of Coronaviruses: SARS CoV-2 response in-line with seasonal coronavirus surrogates", Microbiology Spectrum, vol. 9, issue e00537-21, 2021.
Bradley, R. M., P. M. Marvyn, J. J. Aristiza Henao, E. B. Mardian, S. George, M. G. Aucoin, K. D. Stark, and R. E. Duncan, "Acylglycerophosphate acyltransferase 4 (AGPAT4) is a mitochondrial lysophosphatidic acid acyltransferase that regulates brain phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol levels", Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, vol. 1851, no. 12: Elsevier, pp. 1566–1576, 2015.
Bruder, M. R., S-D. Walji, and M. Gordon Aucoin, "Comparison of CRISPR-Cas9 tools for transcriptional repression and gene disruption in the BEVS", Viruses, vol. 13, issue 1925, 2021.
Bruder, M., and M. Aucoin, CRISPR-dCAS9 for controlling baculovirus replication and increasing production of virus-like particles, 2018.
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Chahal, P. S., M. G. Aucoin, and A. Kamen, "Primary recovery and chromatographic purification of adeno-associated virus type 2 produced by baculovirus/insect cell system", Journal of virological methods, vol. 139, no. 1: Elsevier, pp. 61–70, 2007.
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Dare, E. V., M. Gabriel, A. Begum, M. Sasges, and M. G. Aucoin, "The effect of hydrogen peroxide produced during ultraviolet disinfection of CHO cell culture media", Process Biochemistry, vol. 61: Elsevier, pp. 147–155, 2017.
Delumeau, L-V., H. Asgarimoghaddam, A. T, AJB. Jones, K. Mistry, MG. Aucoin, S. DeWitte-Orr, and KP. Musselman, "Effectiveness of Metal and Metal-Oxide Thin-Film Coatings in Decreasing the Infectious Potential of Human Coronavirus 229E", APL Material, vol. 9, issue 111114, 2021.
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Gabriel, M. A., E. V. Dare, S. M. Meunier, L. J Campbell, M. R. Sasges, and M. G. Aucoin, "Ultraviolet irradiation of trypsin, lysozyme and β-galactosidase: how does UVC affect these enzymes when used as a secondary barrier against adventitious agents?", Vaccine, vol. 37, no. 43: Elsevier, pp. 6518–6525, 2019.
Ganobis, C. M., S. M Al-Abdul-Wahid, S. Renwick, S. Yen, C. Carriero, M. G. Aucoin, and E. Allen-Vercoe, "1D 1H NMR as a Tool for Fecal Metabolomics", Current Protocols in Chemical Biology, vol. 12, no. 3, pp. e83, 2020.
George, S., and M. G. Aucoin, "Characterization of alternative promoters to stagger and control protein expression in the baculovirus-insect cell system: From intracellular reporter proteins to fluorescent influenza virus-like particles", BMC proceedings, vol. 9, no. S9: BioMed Central, pp. P49, 2015.
George, S., A. M. Jauhar, J. Mackenzie, S. Kieβlich, and M. G. Aucoin, "Temporal characterization of protein production levels from baculovirus vectors coding for GFP and RFP genes under non-conventional promoter control", Biotechnology and bioengineering, vol. 112, no. 9: Wiley Online Library, pp. 1822–1831, 2015.
George, S., S. Sokolenko, and M. Gordon Aucoin, "Rapid and cost-effective baculovirus sample preparation method as a viable alternative to conventional preparation for quantitative real-time PCR", Journal of virological methods, vol. 182, no. 1-2: Elsevier, pp. 27–36, 2012.
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Havaei, SM., MG. Aucoin, and A. Jahanian-Najafabadi, "Pseudomonas Exotoxin based immunotoxins: over three decades of efforts on targeting cancer cells with the toxin", Frontiers in Oncology: Cancer Molecular Targets and Therapeutics, vol. 11, issue 781800, 2021.
Henry, O., S. Ansorge, M. Aucoin, R. Voyer, and A. Kamen, "On-line monitoring of cell size distribution in mammalian cell culture processes", IFAC Proceedings Volumes, vol. 40, no. 4: Elsevier, pp. 277–282, 2007.
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Kamen, A. A., M. G. Aucoin, O-W. Merten, P. Alves, Y. Hashimoto, K. Airenne, Y-C. Hu, M. Mezzina, and M. M. van Oers, "An initiative to manufacture and characterize baculovirus reference material", Journal of invertebrate pathology, vol. 107: Elsevier, pp. S113–S117, 2011.
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Lai, Y. P., K. E. K. Sun, C. Moresoli, and M. Aucoin, "DESIGNING AN UNDERGRADUATE FOOD PROCESSING LABORATORY FOR THE UNIVERSITY OF WATERLOO", Proceedings of the Canadian Engineering Education Association (CEEA), 2013.
Lall, D., D. Miscevic, M. R. Bruder, A. Westbrook, M. Aucoin, M. Moo-Young, and C.P. Chou, "Strain engineering and bioprocessing strategies for biobased production of porphobilinogen in Escherichia coli", Bioresource and Bioprocessing, vol. 8, issue 122, 2021.
Liu, M., L. Bauman, C. Lourenco Nogueira, M. G. Aucoin, W. A. Anderson, and B. Zhao, "Antimicrobial Polymeric Composites for High-touch Surfaces in Healthcare Applications", Current Opinion in Biomedical Engineering, 04/2022.
Logan, M., K. Rinas, B.J. McConkey, and M.G. Aucoin, "Vero cells gain renal tubule markers in low-calcium and magnesium chemically defined media", Scientific Reports, vol. 12, pp. 1-13, 2022.
Logan, M., J. Manalil, C. Notte, C. Kearse, S. George, A. Zeiser, P. Farrell, and M. G. Aucoin, "A flow cytometric granularity assay for the quantification of infectious virus", Vaccine, vol. 37, no. 47: Elsevier, pp. 7090–7099, 2019.
Logan, M., and M. Aucoin, Media formulation to support the growth of Vero cells in suspension, 2018.
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Marcali, M., X. Chen, M.G. Aucoin, and C.L. Ren, "Droplet formation of biological non-Newtonian fluid in T-junction generators. I. Experimental investigation", Physical Review E, vol. 105, issue 2, 2022.
Marcali, M., X. Chen, M.G. Aucoin, and C.L. Ren, "Droplet formation of biological non-Newtonian fluid in T-junction generators. II. Model for final droplet volume prediction", Physical Review E, vol. 105, issue 2, 2022.
Meghrous, J., M. G. Aucoin, D. Jacob, P. S. Chahal, N. Arcand, and A. A. Kamen, "Production of recombinant adeno-associated viral vectors using a baculovirus/insect cell suspension culture system: From shake flasks to a 20-L bioreactor", Biotechnology progress, vol. 21, no. 1: American Chemical Society USA, pp. 154–160, 2005.
Mena, J. A., M. G. Aucoin, J. Montes, P. S. Chahal, and A. A. Kamen, "Improving adeno-associated vector yield in high density insect cell cultures", The Journal of Gene Medicine: A cross-disciplinary journal for research on the science of gene transfer and its clinical applications, vol. 12, no. 2: Wiley Online Library, pp. 157–167, 2010.
Meunier, S. M., M. R. Sasges, and M. G. Aucoin, "Evaluating ultraviolet sensitivity of adventitious agents in biopharmaceutical manufacturing", Journal of Industrial Microbiology & Biotechnology, vol. 44, no. 6: Springer Berlin Heidelberg, pp. 893–909, 2017.
Meunier, S. M., B. Todorovic, E. V. Dare, A. Begum, S. Guillemette, A. Wenger, P. Saxena, L. J Campbell, M. Sasges, and M. G. Aucoin, "Impact of dissolved oxygen during UV-irradiation on the chemical composition and function of CHO cell culture media", PloS one, vol. 11, no. 3: Public Library of Science San Francisco, CA USA, pp. e0150957, 2016.
Moussa, H. I., W. Y. Chan, M. Logan, M. G. Aucoin, and T. Y. Tsui, "Limitation in Controlling the Morphology of Mammalian Vero Cells Induced by Cell Division on Asymmetric Tungsten-Silicon Oxide Nanocomposite", Materials, vol. 13, no. 2: Multidisciplinary Digital Publishing Institute, pp. 335, 2020.
Moussa, H. I., M. Logan, K. Wong, Z. Rao, M. G. Aucoin, and T. Y. Tsui, "Nanoscale-Textured Tantalum Surfaces for Mammalian Cell Alignment", Micromachines, vol. 9, no. 9: Multidisciplinary Digital Publishing Institute, pp. 464, 2018.
Moussa, H. I., M. Logan, W. Y. Chan, K. Wong, Z. Rao, M. G. Aucoin, and T. Y. Tsui, "Pattern-Dependent Mammalian Cell (Vero) Morphology on Tantalum/Silicon Oxide 3D Nanocomposites", Materials, vol. 11, no. 8: Multidisciplinary Digital Publishing Institute, pp. 1306, 2018.
Moussa, H. I., M. Logan, G. C. Siow, D. L. Phann, Z. Rao, M. G. Aucoin, and T. Y. Tsui, "Manipulating mammalian cell morphologies using chemical-mechanical polished integrated circuit chips", Science and Technology of advanced MaTerialS, vol. 18, no. 1: Taylor & Francis, pp. 839–856, 2017.
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Nicastro, J., K. Sheldon, F. A. El-zarkout, S. Sokolenko, M. G. Aucoin, and R. Slavcev, "Construction and analysis of a genetically tuneable lytic phage display system", Applied microbiology and biotechnology, vol. 97, no. 17: Springer, pp. 7791–7804, 2013.
Nogueira, C. L., S. J. Boegel, M. Shukla, W. Ngo, L. Jones, and M. G. Aucoin, "Antiviral Activity of Contemporary Contact Lens Care Solutions against Two Human Seasonal Coronavirus Strains", Pathogens, vol. 11, issue 4, 2022.
Nogueira, C. Lourenco, S. J. Boegel, M. Shukla, W. Ngo, L. Jones, and M.G. Aucoin, "The Impact of a Rub and Rinse Regimen on Removal of Human Coronaviruses from Contemporary Contact Lens Materials", Contact Lens and Anterior Eye, 2022.
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Pyne, M. E., S. Sokolenko, X. Liu, K. Srirangan, M. R. Bruder, M. G. Aucoin, M. Moo-Young, D. A. Chung, and P. C Chou, "Disruption of the reductive 1, 3-propanediol pathway triggers production of 1, 2-propanediol for sustained glycerol fermentation by Clostridium pasteurianum", Applied and environmental microbiology, vol. 82, no. 17: American Society for Microbiology, pp. 5375–5388, 2016.
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Quattrociocchi, M., S. J. Boegel, and M. G. Aucoin, "Enhanced characterization of yeast hydrolysate combining acid digestion and 1D-1H NMR targeted profiling", The Canadian Journal of Chemical Engineering, vol. 99, issue S1: John Wiley & Sons, Inc. Hoboken, USA, 2021.
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Shafiee, F., M. G. Aucoin, and A. Jahanian Najafabadi, "Targeted Diphtheria Toxin Based Therapy: A Review Article", Frontiers in microbiology, vol. 10: Frontiers, pp. 2340, 2019.
Sokolenko, S., and M. Aucoin, A correction method for systematic error in metabolomic time-course data, 2016.
Sokolenko, S., M. Quattrociocchi, and M. G. Aucoin, "Identifying model error in metabolic flux analysis–a generalized least squares approach", BMC systems biology, vol. 10, no. 1: BioMed Central, pp. 1–14, 2016.
Sokolenko, S., and M. G. Aucoin, "A correction method for systematic error in 1 H-NMR time-course data validated through stochastic cell culture simulation", BMC systems biology, vol. 9, no. 1: BioMed Central, pp. 1–13, 2015.
Sokolenko, S., E. J. M. Blondeel, N. Azlah, B. George, S. Schulze, D. Chang, and M. G. Aucoin, "Profiling convoluted single-dimension proton NMR spectra: a Plackett–Burman approach for assessing quantification error of metabolites in complex mixtures with application to cell culture", Analytical chemistry, vol. 86, no. 7: American Chemical Society, pp. 3330–3337, 2014.
Sokolenko, S., R. McKay, E. J. M. Blondeel, M. J. Lewis, D. Chang, B. George, and M. G. Aucoin, "Understanding the variability of compound quantification from targeted profiling metabolomics of 1D-1 H-NMR spectra in synthetic mixtures and urine with additional insights on choice of pulse sequences and robotic sampling", Metabolomics, vol. 9, no. 4: Springer, pp. 887–903, 2013.
Sokolenko, S., S. George, A. Wagner, A. Tuladhar, J. M. S. Andrich, and M. G. Aucoin, "Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks", Biotechnology advances, vol. 30, no. 3: Elsevier, pp. 766–781, 2012.
Sokolenko, S., J. Nicastro, R. Slavcev, and M. G. Aucoin, "Graphical analysis of flow cytometer data for characterizing controlled fluorescent protein display on λ phage", Cytometry Part A, vol. 81, no. 12: Wiley Online Library, pp. 1031–1039, 2012.
Sokolenko, S., Y-L. Cheng, and M. Gordon Aucoin, "Getting more from cell size distributions: establishing more accurate biovolumes by estimating viable cell populations", Biotechnology progress, vol. 26, no. 6: Wiley Online Library, pp. 1787–1795, 2010.
Srirangan, K., L. K. Akawi, P. C Chou, M. G. Aucoin, L. E. Stacey, and C. Newton, "Use of a case on metabolically engineered escherichia coli to develop a framework for the design and analysis of bioprocesses", The International journal of engineering education, vol. 33, no. 2: Instituto de Relaciones Internacionales" Daza de Valdes", pp. 751–760, 2017.
Srirangan, K., L. Akawi, X. Liu, A. Westbrook, E. J. M. Blondeel, M. G. Aucoin, M. Moo-Young, and P. C Chou, "Manipulating the sleeping beauty mutase operon for the production of 1-propanol in engineered Escherichia coli", Biotechnology for biofuels, vol. 6, no. 1: BioMed Central, pp. 139, 2013.
Suh, YH., B. Jung Kim, K. Chiu Tam, and M. Gordon Aucoin, "Detection and characterization of hemoglobin dissociation and aggregation using microcalorimetry", Journal of Thermal Analysis and Calorimetry, vol. 115, no. 3: Springer, pp. 2159–2169, 2014.
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Thompson, C. M., M. G. Aucoin, and A. A. Kamen, "Production of virus-like particles for vaccination", Baculovirus and Insect Cell Expression Protocols: Springer, pp. 299–315, 2016.
Thompson, C. M., J. Montes, M. G. Aucoin, and A. A. Kamen, "Recombinant protein production in large-scale agitated bioreactors using the baculovirus expression vector system", Baculovirus and Insect Cell Expression Protocols: Springer, pp. 241–261, 2016.
Thompson, C. M., E. Petiot, M. G. Aucoin, O. Henry, and A. A. Kamen, "Developing a production process for influenza VLPs: a comparison between HEK 293SF and Sf9 production platforms", BMC proceedings, vol. 7, no. S6: BioMed Central, pp. P22, 2013.
Thompson, C. M., E. Petiot, A. Mullick, M. G. Aucoin, O. Henry, and A. A. Kamen, "Critical assessment of influenza VLP production in Sf9 and HEK293 expression systems", BMC biotechnology, vol. 15, no. 1: BioMed Central, pp. 31, 2015.
Todorovic, B., E. V. Dare, A. Begum, S. R. Guillemette, A. Wenger, P. Saxena, L. J Campbell, M. Sasges, M. Aucoin, and others, Impact of dissolved oxygen during UV-irradiation on the chemical composition and function of CHO cell culture media: Public Library of Science, 2016.
Transfiguracion, J., J. A. Mena, M. G. Aucoin, and A. A. Kamen, "Development and validation of a HPLC method for the quantification of baculovirus particles", Journal of Chromatography B, vol. 879, no. 1: Elsevier, pp. 61–68, 2011.
Tsui, T. Y., M. Logan, H. I. Moussa, and M. G. Aucoin, "What’s Happening on the Other Side? Revealing Nano-Meter Scale Features of Mammalian Cells on Engineered Textured Tantalum Surfaces", Materials, vol. 12, no. 1: Multidisciplinary Digital Publishing Institute, pp. 114, 2019.
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Varga, JFA.,, G. Cheng, D. Min, MG. Aucoin, AC. Doxey, and B. Dixon, "Identification and characterization of a novel peptide from rainbow trout (Oncorhynchus mykiss) with antimicrobial activity against Streptococcus iniae", Developmental and Comparative Immunology, vol. 137, issue 104518, pp. 1-7, 2022.
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Walji, S-D., M. R. Bruder, and M.G. Aucoin, "Virus Matrix Interference on Virucidal Activity of High-Touch Surfaces Designed to Prevent Hospital-Acquired Infections", Antimicrobial Resistance and Infection Control, vol. 10, issue 133, 2021.
Walji, S-D., and M. G. Aucoin, "A critical evaluation of current protocols for self-sterilizing surfaces designed to reduce viral nosocomial infections", American Journal of Infection Control: Mosby, 2020.
Wulff, D., A. Chan, Q. Liu, F. X. Gu, and M. G. Aucoin, "Characterizing internal cavity modulation of corn starch microcapsules", Heliyon, vol. 6, no. 10: Elsevier, pp. e05294, 2020.
Wulff, D., M. G. Aucoin, and F. X. Gu, "Helium Ion Microscopy of Corn Starch", Starch-Stärke, vol. 72, issue 11-12, pp. 1900267, 11/2020.
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Yen, S., E. Bolte, M. Aucoin, and E. Allen-Vercoe, "Metabonomic Evaluation of Fecal Water Preparation Methods: The Effects of Ultracentrifugation", Current Metabolomics, vol. 6, no. 1: Bentham Science Publishers, pp. 57–63, 2018.
Yen, S., J. A. K. McDonald, K. Schroeter, K. Oliphant, S. Sokolenko, E. J. M. Blondeel, E. Allen-Vercoe, and M. G. Aucoin, "Metabolomic analysis of human fecal microbiota: a comparison of feces-derived communities and defined mixed communities", Journal of proteome research, vol. 14, no. 3: American Chemical Society, pp. 1472–1482, 2015.
Yen, S., S. Sokolenko, B. Manocha, A. Patras, F. Daynouri-Pancino, E. J. M. Blondeel, M. Sasges, and M. G. Aucoin, "Treating cell culture media with UV irradiation against adventitious agents: Minimal impact on CHO performance", Biotechnology progress, vol. 30, no. 5, pp. 1190–1195, 2014.