Citation:
Narayanan, N. , & Chou, C. P. . (2009). Alleviation of Proteolytic Sensitivity To Enhance Recombinant Lipase Production in Escherichia coli. Applied and Environmental Microbiology, 75, 5424-5427.
Abstract:
Two amino acids, Leu149 and Val223, were identified as proteolytically sensitive when Pseudozyma antarctica lipase (PalB) was heterologously expressed in Escherichia coli. The functional expression was enhanced using the double mutant for cultivation. However, the recombinant protein production was still limited by PalB misfolding, which was resolved by DsbA coexpression.
Notes:
Narayanan, Niju Chou, C. Perry
