Citation:
Chou, C. P. , & Tseng, J. H. . (2000). Effect of carbon source on inclusion body formation upon overproduction of periplasmic penicillin acylase in Escherichia coli. Journal of the Chinese Institute of Chemical Engineers, 31, 219-224.
Abstract:
The effect of carbon supplementation on penicillin acylase (PAC) production and inclusion body formation was investigated. The host effect was also examined by comparing culture performances using HB101 or its derived mutant, MD Delta P7, as the expression host. Among several carbons tested, glycerol was found to be optimum for PAC production. PAC activity was increased and the amount of inclusion bodies was reduced upon glycerol supplementation. The ef feet of carbon source on inclusion body formation appears to be strain-dependent. With the optimization on the culture parameters (i.e., host and carbon effects), volumetric PAC activity could be significantly improved at more than fourfold that of the control experiment. This information is useful for developing expression strategies for optimization of PAC production.
Notes:
Chou, CP Tseng, JH
