Publications

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[ Author(Desc)] Title Type Year
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Narayanan, N. , Khan, M. , & Chou, C. P. . (2010). Enhancing functional expression of heterologous lipase B in Escherichia coli by extracellular secretion. Journal of Industrial Microbiology & Biotechnology, 37, 349-361.
Narayanan, N. , & Chou, C. P. . (2009). Alleviation of Proteolytic Sensitivity To Enhance Recombinant Lipase Production in Escherichia coli. Applied and Environmental Microbiology, 75, 5424-5427. 2009_alleviation_of_proteolytic_sensitivity_to_enhance_recombinant_lipase_production_in_escherichia_coli.pdf
Narayanan, N. , Follonier, S. , & Chou, C. P. . (2008). In vivo monitoring and alleviation of extracytoplasmic stress to recombinant protein overproduction in the periplasm of Escherichia coli. Biochemical Engineering Journal, 42, 13-19.
Narayanan, N. , & Chou, C. P. . (2008). Periplasmic chaperone FkpA reduces extracytoplasmic stress response and improves cell-surface display on Escherichia coli. Enzyme and Microbial Technology, 42, 506-513.
Narayanan, N. , & Chou, C. P. . (2008). Physiological improvement to enhance Escherichia coli cell-surface display via reducing extracytoplasmic stress. Biotechnology Progress, 24, 293-301.
Narayanan, N. , Hsieh, M. Y. , Xu, Y. L. , & Chou, C. P. . (2006). Arabinose-induction of lac-derived promoter systems for penicillin acylase production in Escherichia coli. Biotechnology Progress, 22, 617-625.
Narayanan, N. , Xu, Y. L. , & Chou, C. P. . (2006). High-level gene expression for recombinant penicillin acylase production using the araB promoter system in Escherichia coli. Biotechnology Progress, 22, 1518-1523.
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Orr, V. , Zhong, L. Y. , Moo-Young, M. , & Chou, C. P. . (2013). Recent advances in bioprocessing application of membrane chromatography. Biotechnology Advances, 31, 450-465. 2013_recent_advances_in_bioprocessing_application_of_membrane_chromatography.pdf
Orr, V. , Scharer, J. , Moo-Young, M. , Honeyman, C. H. , Fenner, D. , Crossley, L. , Suen, S. Y. , et al. (2012). Simultaneous clarification of Escherichia coli culture and purification of extracellularly produced penicillin G acylase using tangential flow filtration and anion-exchange membrane chromatography (TFF-AEMC). Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 900, 71-78.
Orr, V. , Scharer, J. , Moo-Young, M. , Honeyman, C. H. , Fenner, D. , Crossley, L. , Suen, S. Y. , et al. (2012). Integrated development of an effective bioprocess for extracellular production of penicillin G acylase in Escherichia coli and its subsequent one-step purification. Journal of Biotechnology, 161, 19-26.
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Pan, K. L. , Hsiao, H. C. , Weng, C. L. , Wu, M. S. , & Chou, C. P. . (2003). Roles of DegP in prevention of protein misfolding in the Periplasm upon overexpression of penicillin acylase in Escherichia coli. Journal of Bacteriology, 185, 3020-3030. 2003_roles_of_degp_in_prevention_of_protein_misfolding_in_the_periplasm_upon_overexpression_of_penicillin_acylase_in_escherichia_coli.pdf
Pyne, M. E. , Sokolenko, S. , Liu, X. J. , Srirangan, K. , Bruder, M. R. , Aucoin, M. G. , Moo-Young, M. , et al. (2016). Disruption of the Reductive 1,3-Propanediol Pathway Triggers Production of 1,2-Propanediol for Sustained Glycerol Fermentation by Clostridium pasteurianum. Applied and Environmental Microbiology, 82, 5375-5388. 2016_disruption_of_the_reductive_13-propanediol_pathway.pdf
Pyne, M. E. , Liu, X. J. , Moo-Young, M. , Chung, D. E. A. , & Chou, C. P. . (2016). Genome-directed analysis of prophage excision, host defence systems, and central fermentative metabolism in Clostridium pasteurianum. Scientific Reports, 6. 2016_genome-directed_analysis_of_prophage_excision_host_defence_systems_and_central_fermentative_metabolism_in_clostridium_pasteurianum.pdf
Pyne, M. E. , Bruder, M. R. , Moo-Young, M. , Chung, D. A. , & Chou, C. P. . (2016). Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium. Scientific Reports, 6. 2016_harnessing_heterologous_and_endogenous_crispr-cas_machineries_for_efficient_markerless_genome_editing_in_clostridium.pdf
Pyne, M. E. , Moo-Young, M. , Chung, D. E. A. , & Chou, C. P. . (2015). Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli. Applied and Environmental Microbiology, 81, 5103-5114. 2015_coupling_the_crispr_cas9_system_with_lambda_red_recombineering_enables_simplified_chromosomal_gene_replacement_in_escherichia_coli.pdf
Pyne, M. E. , Utturkar, S. , Brown, S. D. , Moo-Young, M. , Chung, D. A. , & C. Chou, P. . (2014). Improved Draft Genome Sequence of Clostridium pasteurianum Strain ATCC 6013 (DSM 525) Using a Hybrid Next-Generation Sequencing Approach. Genome announcements, 2, e00790-14.
Pyne, M. E. , Moo-Young, M. , Chung, D. A. , & Chou, C. P. . (2014). Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: chromosomal gene disruption of the endogenous CpaAI restriction enzyme. Biotechnology for Biofuels, 7. 2014ex1.pdf
Pyne, M. E. , Bruder, M. , Moo-Young, M. , Chung, D. A. , & Chou, C. P. . (2014). Technical guide for genetic advancement of underdeveloped and intractable Clostridium. Biotechnology Advances, 32, 623-641. 2014_technical_guide_for_genetic_advancement_of_underdeveloped_and_intractable_clostridium.pdf
Pyne, M. E. , Moo-Young, M. , Chung, D. A. , & Chou, C. P. . (2013). Development of an electrotransformation protocol for genetic manipulation of Clostridium pasteurianum. Biotechnology for Biofuels, 6. 2013_development_of_an_electrotransformation_protocol_for_genetic_manipulation_of_clostridium_pasteurianum.pdf
Pyne, M. E. , Sukhija, K. S. , & Chou, C. P. . (2010). Strain improvement of Escherichia coli to enhance recombinant protein production. In Manual of Industrial Microbiology and Biotechnology (3rd ed., pp. 273-286). ASM Press. asm_mimb3_ch19.pdf

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