This paper presents a novel approach to automated generation of driving lines from mobile laser scanning (MLS) point cloud data. The proposed method consists of three steps: road surface segmentation, road marking extraction and classification, and driving line generation. The voxel-based upward-growing algorithm was firstly used to extract ground points from the raw MLS point clouds followed by segmentation of road surface using a regiongrowing algorithm. Then, the statistical outlier removal filter was applied to separate and refine the road marking points followed by extracting and classifying the lane markings based on the geometric features of different road markings using empirical hierarchical decision trees. Finally, land node structures were constructed followed by generation of driving lines using a curve-fitting algorithm. The proposed method was tested on both circular road sections and irregular intersections. The smoothing spline curve fitting model was tested on the circular road sections, while the Catmull-Rom spline with five control points was used to generate the driving lines at road intersections. The overall performance of the proposed algorithms is promising with 96.0% recall, 100.0% precision, and 98.0% F1-score for the lane marking extraction specifically. Most significantly, the validation results demonstrate that the driving lines can be effectively generated within 20 cm-level localization accuracy at an average of 3.5% miscoding using MLS point clouds, which meets the requirement of localization accuracy of fully autonomous driving functions. The results demonstrate the proposed methods can successfully generate road driving lines in the test datasets to support the development of high-definition maps.