Liquid Chromatography
Thermo Scientific Orbitrap coupled to Thermo UHPLC Vanquish system
This instrument is equipped with atmospheric pressure ionization (API) source available as heated electrospray ionization (h)ESI and contains a C-trap for short-pulsed injection into the orbitrap mass analyzer that uses fourier transform to provide highly resolved mass analysis. Coupled with a quaternary pump UHPLC with pressure limits up to 1000 bar, it is used mainly for metabolomic investigations. Purchased with the air of Canada Foundations for Innovations funds.
Location: InFAReL LC Lab (ESC-223A)
Contact: Will Jiang (rwjiang@uwaterloo.ca)
Thermo TSQ Vantage LC-MS
TSQ Vantage triple quadrupole mass spectrometer interfaced with a Thermo Vanquish liquid chromatography system. Purchased with the aid of Canada Foundation for Innovation funds.
Location: InFAReL LC Lab (ESC-223A)
Contact: Daniel Rickert (darickert@uwaterloo.ca)
Applied Biosystems API 4000 MS/MS
This instrument is equipped with atmospheric pressure ionization (API) source available as Turbo ion spray (TIS) for more efficient spray nebulization. It is a triple quadrupole mass analyzer which is optimal for targeted analysis of various types of complex molecules from xenobiotics to peptides and as such, serves as the laboratory workhorse. Donated from Merk & Co. Inc, New Jersey.
Location:InFAReL GC Lab (ESC-223)
Contact: Emir Nazdrajic (enazdrajic@uwaterloo.ca)
Thermo TSQ Quantiva Triple-Quadrupole MS with Dionex UltiMate 3000 RS UHPLC System
Waters Xevo G2-S QTof with Acquity UPLC M-Class System
A hybrid, quadrupole, orthogonal acceleration, time-of-flight (TOF) mass spectrometer with a multi-mode, dual-orthogonal atmospheric pressure Spray ionization source, operated by MassLynx software. The UPLC system is capable of micro- and nano-scale separations. The entire system has been kindly provided to our lab by Waters Corporation as a seed instrument.
Location InFAReL LC Lab (ESC 223)
Contact: Nando Rosales (hrosalesuwaterloo.ca)
Shimadzu LCMS-8060 with LC-30AD
Shimadzu LCMS-8060 a triple quadrupole mass spectrometer interfaced with a Shimadzu LC-30AD on loan from Shimadzu Corporation.
Location InFAReL LC Lab (ESC 223A)
Contact: Emir Nazdrajic (enazdrajic@uwaterloo.ca)
Gas Chromatography
Agilent 6890A-5973N, GC-MS
This Agilent GC-MS single quadrupole uses electron impact for ionization. The instrument is fitted with a Gerstel brand autosampler with multi-function capabilities. The autosampler arm can be adjusted for regular needle injection, SPME using the fiber configuration, as well as for membrane or other SPME extraction forms using the thermal desorption unit (TDU). This device, installed onto a cooled injection system (CIS), allows for the desorption of analytes from the extraction material at temperatures up to 300°C, which are then trapped by the CIS using liquid nitrogen. The CIS is then rapidly heated to release all the trapped analytes at once onto the column, resulting in a very sharp injection.
Location: InFAReL GC Lab (ESC-223)
Contact: Khaled Murtada (kmurtada@uwaterloo.ca)
HP 6890 GC 5973 MS
This HP instrument has been a lab workhorse since before HP was purchased by Agilent. The single quadrupole with an electron impact source offers reliable ionization for comparison searches using NIST published libraries.
Fitted with the new PAL robotic tool exchange (RTC) autosampler from CTC Analytics, this system can do nearly anything. The tool exchanger allows for automated changing of sampling style within a single sequence; as an example, it can perform automated sample preparation involving dilutions using the syringe tool, followed by an SPME extraction.
Location: ESC-223 (InFaRel GC Lab)
Contact: Khaled Murtada (kmurtada@uwaterloo.ca)
Agilent 5890 Series II GC-FID
Agilent 5890 series II GC equipped with a flame ionization detector. Purchased from McMaster University.
Location: C2 Main Lab (C2-162A)
Contact: Khaled Murtada (kmurtada@uwaterloo.ca)
Thermo Trace GC Ultra GC-FID,NPD
Thermo Trace GC Ultra system with flame ionization detector (FID), nitrogen phosphorus detector (NPD), and TriPlus autosampler.
Purchased with the aid of Canada Foundation for Innovation funds.
Location: InFAReL GC Lab (ESC-222)
Contact: Khaled Murtada (kmurtada@uwaterloo.ca)
Varian CP-3800 GC-FID,TSD,ECD
Varian CP-3800 GC system with flame ionization detector (FID), thermionic specific detector (TSD), and electron capture detector (ECD) along with the CTC Analytics PAL autosampler.
Purchased with the aid of Canada Foundation for Innovation funds and support from Varian.
Location: C2 Main Lab (C2-162A)
Contact: Khaled Murtada (kmurtada@uwaterloo.ca)
Torion Tridion-9 portable GC-MS
Torion Technologies Tridion-9 field portable GC-MS with a miniaturized toroidal ion trap mass analyzer. Purchased with the aid of Ontario Research Fund and NSERC equipment fund with support from Torion.
Location: C2 Main Lab (C2-162A)
Contact: Khaled Murtada (kmurtada@uwaterloo.ca)
Agilent 7890 GC 5977A MS
This recently purchased GC-MS offers greater sensitivity than those before it. Also a single quadrupole with electron impact, it is one of Agilent’s newest lines of GC-MS products.
Fitted with a Gerstel MPS Robotic Pro, the same TDU and CIS equipment as in the Agilent 6890 are also installed here. With the most recent version of the autosampler, individual tools have been designed for each sampling mode, removing the requirement for the user to manually change the configuration of the sampling head.
Location ESC-223 (InFARel Gc Lab)
Contact: Khaled Murtada (kmurtada@uwaterloo.ca)
Other Instrumentation
Capillary Electrophoresis: Beckman P/ACE™ MDQ
The Beckman P/ACE™ MDQ is a capillary electrophoresis (CE) system. It includes Methods Development System configured with selectable-wavelength UV/Vis (200, 214, 254 and 280 nm filters included) detector, Sample Temperature Controlled and 32 Karat™ Software configured on a desktop controller. Purchased with the aid of Canada FedDev funds.
Location: InFAReL GC Lab (ESC-223)
Contact: Khaled Murtada (kmurtada@uwaterloo.ca)
Capillary Electrophoresis: Convergent Bioscience iCE 280
Convergent Bioscience iCE 280 for capillary isoelectric focusing (CIEF) with whole column imaging detection (WCID), without a mobilization phase. Purchased with the aid of NSERC Industrial Research Chair funds and support from Convergent Bioscience.
Location: InFAReL GC Lab (ESC-223)
Contact: Khaled Murtada (kmurtada@uwaterloo.ca)
Particle Generator/Sizer: TSI SPMS 3936 Particle Generator and Sizer
The TSI SPMS 3936 Particle Generator-Scanning Mobility Particle Sizer with Electrostatic Classifier (Series 3080) is a system that measure the size distribution of particles chemicals (TICs).
Location: C2 Main Lab (C2-162A)
Contact: Shakiba Zeinali (szeinali@uwaterloo.ca)
96-Blade Concept:PAS Concept-96 Autosampler
Concept 96 Autosampler: This robotic 96-well plate workstation is designed for high-throughput sampling and sample preparation using the 96-array SPME brush/blade system.
Acquired with the support of PAS Instruments.
Location: InFAReL LC Lab (ESC-223A)
Contact: Emir Nazdrajic (enazdrajic@uwaterloo.ca )
Thermal Desorption: Torion High Volume Desorber
The Torion developed high volume desorption module is designed to thermally desorb standard 3.5 inch sorbent tubes into a Custodian 19-gauge needle trap device for use with the Tridion-9 GC/MS. Donated by Torion Technologies Inc.
Location: C2 Main Lab (C2-162A)Contact: Shakiba Zeinali (szeinali@uwaterloo.ca)
Raman Spectrometer
Homemade Raman spectrometer is specifically designed for detection of analytes in the SPME probe.
The Raman spectrometer based on CCD camera PIXIS 256 (Princeton Instruments), high-aperture
transmittance spectrograph (P;P Optica), and integrated Raman head (Innovative Photonic Solutions).
Specification: single-mode diode-laser excitation at 785 nm; spectral range 120 - 2750 cm -1 ; spectral
resolution 5 cm -1 FWHM.
Location : ESC-141
Contact: Khaled Murtada (kmurtada@uwaterloo.ca)
