As a PhD candidate at University of Waterloo I work on the application of solid phase microextraction (SPME) for the targeted and untargeted analysis of biological samples. In terms of targeted analysis, I have participated in the development of SPME methods for the analysis of prohibited substances in plasma, whole blood and urine samples. My research scope also involves new developments and approaches to manufacture SPME devices compatible with LC-MS methods. I am also working on collaborative projects with Toronto General Hospital where we are investigating the applicability of SPME coupled to LC-HRMS for untargeted analysis of different tissues. Part of my research also involves the investigation of complex matrices and biding phenomena using SPME.
As a PhD candidate at Prof. Janusz Pawliszyn’s research group, I am currently working on a collaborative project with thoracic and general surgeons at Toronto General Hospital (TGH) on the development of a rapid diagnostic tool for targeted in-vivo, ex-vivo and on-site analysis. This technology is based on the direct coupling of biocompatible Solid Phase Micro Extraction (SPME) devices to mass spectrometry instruments using different ionization techniques such as Direct Analysis in Real Time (DART), Desorption Electro Spray Ionization (DESI), nano-Electro Spray Ionization (nano-ESI), and Coated Blade Spray (CBS). My PhD research thesis is mainly focused on the improvement of the technology in order to provide accurate, fast, and low-cost assays of biological fluids and tissue, allowing medical doctors and surgeons to individualize patient’s treatment.
My primary research is focused on the development and applications of thin film microextraction (TFME) technologies for use with portable GC-MS instrumentation for on-site analysis. This research is a collaborative project with Torion Technologies Inc. and will be performed on the Tridion-9 GC-TMS (toroidal mass spectrometer ). My proposed research will aim to prepare and evaluate a variety of particle loaded, thin film membranes and develop an interface to allow these membranes to be used in conjunction with portable instrumentation for on-site analysis.
I work on the development of novel SPME-MS interfaces and multiple projects related to the application of SPME on the clinical settings!
My research focuses on the application of Solid Phase Microextraction (SPME) in in vivo metabolomics study. SPME has great potential in monitoring metabolism process as a fast, simple and high-throughput sample preparation method. The tremendous amount of data from SPME is valuable for diving in the metabolomic process, which requires delicate data mining method. Currently, I am working on removal of the the patterned noise other than biological signals in the application of SPME in metabolomics study. Further I will go into the details of the statistical properties of such omics related data, simulate and model them for SPME-based metabolomics.
As a Postdoctoral Fellow at University of Waterloo I work on the development of coatings for solid phase microextraction (SPME). These studies include synthesis, functionalization and characterization of materials and their application in the determination of compounds of interest from environmental and biological samples. The primary focus of the developed materials will be miniaturization of coatings and application in various formats of SPME like Coated Blade Spray, In-tube, On-Tip and Thin film microextraction (TFME) etc. My current research involves materials chemistry, mass spectrometry, sample preparation, method development and chromatography.
My research focuses on using solid phase microextraction for therapeutic drug monitoring and untargeted analysis in vivo and ex vivo. I am currently involved in two projects that are both in collaboration with Toronto General Hospital wherein my responsibilities include but are not limited to SPME method and protocol development for investigating various biological matrices for the purposes of monitoring drug concentrations and drug efficacy.
My research interests include application and improvement of SPME-based methodologies for brain neurochemistry studies and forensic science.
My current work focuses on the development and improvement of techniques for comprehensive in-vivo and post-mortem brain studies including direct SPME-MS coupling enabling rapid analysis with increased sensitivity and selectivity as well as obtaining information about spatial distribution of both endogenous compounds and xenobiotics in various brain structures when employed in MS-imaging mode.
Additionally, my work will involve introducing the SPME-based technologies to the post-mortem forensic toxicological analyses, where the time factor is of greatest importance. As SPME probes possess the ability to preserve the extracted compounds, this approach will allow obtaining valuable toxicological information from tissues subjected to the fastest rate of decay process, such as brain tissue.
Currently I am involved in the project which accounts for the development of SPME fiber for in-vivo extraction of beta amyloids from blood plasma. Besides, my mathematical modeling works involve in understanding the radial diffusion with different SPME geometries
SPME represents a great tool for forensic science due to its rapid application and high ability in sample preservation which are of uttermost importance in law enforcement. My current research is focused on in vivo applications of SPME in metabolomics with a specific focus in targeting xenobiotic compounds. The ability of SPME to detect such compounds shows a great potential for its use in forensic toxicology. I will also be working on the optimization of SPME for use with Raman Spectroscopy in order to provide more rapid sample analysis.
The improper application of veterinary drugs in food-producing animals and failure to comply with withdrawal times can lead to the presence of drug residue in the edible tissue of the treated animal. These residues, even at low concentrations, may pose health risk to humans consuming the products of the treated animals. The primary focus of my PhD project is to develop and validate a fully automated, high-throughput analytical method for the determination of multi-class veterinary drug residues in animal tissues, using solid-phase microextraction (SPME) and ultra high pressure liquid chromatography-mass spectrometry (UHPLC-MS).
My research focuses on development of sample preparation methods for aerosol analysis. These include the development of alternative sorbents for needle trap devices (NTDs), thedevelopment of sorbent and filter packing procedure for NTDs and the applications of NTDs for on-site analysis.
My M.Sc research in the Pawliszyn research group focuses on the development and optimization of ambient ionization mass spectrometry techniques such as coated blade spray (CBS) for quantitative analysis of biofluids. By using techniques such as CBS we can improve selectivity of target compounds, reduce analysis time and lower detection and quantitation limits compared to more traditional approaches. Furthermore, I am also responsible for validating new SPME coatings for use in bioanalysis and metabolomics studies
As a M.Sc student in Professor Pawliszyn's research group my project is going to be focus on the development and use of new matrix compatible coatings for SPME devices, intended for analysis of food matrices. In terms of applications, I will focus on both targeted and untargeted analysis by using gas-chromatography (both unidimensional and comprehensive two-dimensional gas-chromatography) hyphenated to mass spectrometry.
My work involves the development of testing applications and analytical methods involving a GC-MS setup in order to sample complex matrices. I was involved in studying methods to detect date rape drugs in beverage matrices and bodily fluids at low concentrations. Currently, I am working on a joint project with Toronto’s Sick Kids Hospital to test for volatile compounds in blood involving epilepsy treatments. In future work, I plan to develop methods of testing for pesticides in commercial tea leaves, as well as for testing TFME membranes in complex environmental matrices.
My research, as an M.Sc. student, is primarily focused on the development of a solid phase micro-extraction (SPME) methodology for untargeted analysis of lipids in biological samples, such as plasma. In addition, I will be working on a collaborative project with Toronto General Hospital, regarding the application of SPME for untargeted analysis after organ transplant.
My current research involves in silico studies of solid phase microextraction used to predict behavior of analytes and other sample components in various matrices. The purpose of these studies is to provide insight prior to real-time experiments. Moreover, I am involved in the development of novel SPME-MS interfaces.
As part of my research project I plan to work on the extraction of pesticides in drinking water by means of polytetrafluoroethylene amorphous fluoroplastics (PTFE AF 2400)-based thin-film membranes loaded with adsorptive particles. The incorporation of appropiate sorbents will be investigated in light of covering analytes with a broad range of hydrophobicities and molecular weight demostrating its compatibility for thermal and solvent-assisted desorption as well.
The focus of my undergraduate research is the development of ambient ionization techniques. Specifically, the development of novel coated blade spray (CBS) applications as well as further optimization of the technique. Developments being pursued include complete technique automation, untargeted compound analysis, and in-field sampling.