The present work documents the development and validation of a portable fluorometer designed to detect and quantify the fluorescence of DOX that has been extracted using SPME probes. This project is a continuation of our pioneering works on in vivo SPME. The developed fluorometer is intended for use in monitoring DOX concentrations in perfusate solution and the lung tissue during IVLP in a hospital setting. Therefore, in configuring the fluorometer and determining its specifications, we opted to use SPME fibers with a biocompatible coating and the in vivo SPME protocol, which have both been previously tested and optimized for DOX analysis.9,35 In addition, we studied the fluorescence features of DOX when adsorbed to the SPME coating. The protocol for SPME sampling and fluorescence measurements was modified to take these features into account. Finally, we performed a feasibility test using the fluorometer to quantitate doxorubicin concentrations in perfusate solution and the homogenized lamb lung tissue.
V. Galievsky and J. Pawliszyn. Fluorometer for Screening of Doxorubicin in Perfusate Solution and Tissue with Solid-Phase Microextraction Chemical Biopsy Sampling. Analytical Chemistry 92 (2020) 13025–13033. https://dx.doi.org/10.1021/acs.analchem.0c01905