In this study, Bio-SPME is used to monitor DOX and its metabolites, as well as small endogenous molecules, present within the system over the course of IVLP. The current work presents the determination and quantification of DOX in the following settings: (i) an in vivo pig model over various drug doses as a proof-of-concept and (ii) in vivo IVLP human clinical trials. The work also demonstrates that the SPME chemical biopsy captures other low molecular weight compounds, such as potential markers of drug toxicity or organ stress over the course of IVLP.
B. Bojko, N. Looby, M. Olkowicz, A. Roszkowska, B. Kupcewicz, P. R. des Santos, K. Ramadan, S. Keshavjee, T. K. Waddell, G. Gómez-Ríos, M. Tascon, K. Goryński, M. Cypel, J. Pawliszyn. Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion. Journal of Pharmaceutical Analysis. https://doi.org/10.1016/j.jpha.2020.08.011