The objective of this work was to introduce in vivo SPME sampling as a new method to accurately measure detailed oxylipin profiles in the brains of living, awake rats. This new approach successfully eliminates post-mortem changes in oxylipin concentrations, allows time-course monitoring of their concentrations with high spatial resolution, and can be performed using the same experimental set-up as in vivo MD, which is considered the gold standard method in neuroscience.
A. Napylov, N. Reyes-Garces, G. Gomez-Rios, M. Olkowicz, S. Lendor, C. Monnin, B. Bojko, C. Hamani, J Pawliszyn, and D. Vuckovic. In Vivo Solid‐Phase Microextraction for Sampling of Oxylipins in Brain of Awake, Moving Rats. Angew. Chem. Int. Ed. 2019, 58, 2–9. https://doi.org/10.1002/anie.201909430