Reference: Albertano, P., G. Pinto, S. Santisi and R. Taddei. (1981). Spermatozopsis acidophila Kalina (Chlorophyta, Volvocales), a little known alga from highly acidic environments. Giornale Botánico Italiano, 115, 65-76
| Stock | Substance | Concentration (g/L) | Amount added to medium (mL/L) |
|---|---|---|---|
| 1. | (NH4)2SO4 | 130 | 10 mL |
| 2. | MgSO4•7H2O | 30 | 10 mL |
| 3. | KH2PO4 | 30 | 10 mL |
| 4. | NaCl | 10 | 10 mL |
| 5. | CaCl2•2H2O | 2 | 10 mL |
| 6. | H3BO3 | 0.6 | 10 mL |
| 7. | Albertano metal solution | See below | 10 mL |
Albertano metal solution
| Substance | Concentration (g/L) |
|---|---|
| 1. FeCl3•6H2O | 0.54 g |
| 2. MnCl2•4H2O | 0.20 g |
| 3. ZnCl2 | 0.14 g |
| 4. CuSO4•5H2O | 0.10 g |
| 5. Na2MoO4•2H2O | 0.05 g |
| 6. CoCl2•6H2O | 0.05 g |
Final pH of medium is adjusted to 1.0 using approximately 4.1 mL of H2SO4 (concentrated). If preparing solid media, make the medium with 1.5% agar but without H2SO4. Let the agar set and then cover the surface with a thin coating of 20% H2SO4(conc.). Inoculate the medium after a few days. This medium is used for Dunaliella acidophila (= Spermatozopsis acidophila).