Albertano concentrate | Canadian Phycological Culture Centre

Reference: Albertano, P., G. Pinto, S. Santisi and R. Taddei. (1981). Spermatozopsis acidophila Kalina (Chlorophyta, Volvocales), a little known alga from highly acidic environments. Giornale Botánico Italiano, 115, 65-76

Stock	Substance	Primary stock (g/L)	Volume used to prepare 90 mL concentrate	Concentrated stock (g/L)	Final concentration in medium (mg/L) (adding 90 mL/L)
	Purified H₂O	–	30 mL	–	–
1.	(NH₄)₂SO₄	130	10 mL	14.4	1300
2.	MgSO₄•7H₂O	30	10 mL	3.3	300
3.	KH₂PO₄	30	10 mL	3.3	300
4.	NaCl	10	10 mL	1.1	100
5.	CaCl₂•2H₂O	2	10 mL	0.2	20
6.	H₃BO₃	0.6	10 mL	0.07	6
7.	Albertano metal solution^a	See below	– ^a	– ^a	See below

^a NOTE: The Albertano Metal Solution (Stock 7) that is normally added to Albertano medium is removed from the concentrate as it precipitates out. Mix this stock well and add separately during preparation of the medium.

Albertano metal solution

Substance	Primary stock (g/L)	Final concentration in medium (mg/L)
1. FeCl₃•6H₂O	0.54 g	5.4
2. MnCl₂•4H₂O	0.20 g	2.0
3. ZnCl₂	0.14 g	1.4
4. CuSO₄•5H₂O	0.10 g	1.0
5. Na₂MoO₄•2H₂O	0.05 g	0.5
6. CoCl₂•6H₂O	0.05 g	0.5

To prepare concentrate:

To make the concentrate, combine stock solutions 1 - 6, in the order listed above, to make 90 mL total volume. Concentrate may be stored in the fridge in the dark until needed.

To prepare medium:

The final concentrations shown above are to prepare a 100% Albertano medium by adding 90 mL of the concentrate plus 10 mL of Stock 7 (trace metals) to 900 mL of purified water(e.g., distilled, Nanopure, Milli-Q). Stir, pH adjust to 1 if necessary with concentrated H₂SO₄ (initial pH will likely be ~4.5-5). If sterile medium is needed, then the final medium may be filter-sterilized through a 0.22 um filter and dispensed aseptically into sterile vessels or autoclaved directly in the vessels. Prepared medium may be stored in the fridge in the dark until needed.