Marc Aucoin, PEng (He/Him)

• Virus propagation
• Viral vector
• Virus-like particle
• Vaccine production
• Infection
• Gene delivery
• Animal cell culture
• Biochemical & Biomedical
• Mammalian cell
• Metabolism
• Bioprocess development
• Monoclonal Antibody Production and Modification
• Biologic Production
• Baculovirus Expression Vectors
• Serological Assays
• Cell Culture Monitoring
• Modeling and Simulation
• Synthetic Biology

• 2007, Doctorate Chemical Engineering, Ecole Polytechnique de Montreal, Canada
• 2003, Master's Chemical Engineering, University of Waterloo, Canada
• 2000, Bachelor's Chemical Engineering, University of Waterloo, Canada

• 2022 Faculty of Engineering Outstanding Performance Award
• 2018 Faculty of Engineering Distinguished Performance Award for Outstanding Contribution to Teaching, Scholarship and Service
• 2016 Faculty of Engineering Outstanding Performance Award
• 2011 Faculty of Engineering Distinguished Performance Award for Outstanding Contribution to Teaching, Scholarship and Service

• 2021- Accociate Chair, Operations
• 2020-2021 Interim Chair, Department of Chemical Engineering
• 2020 Associate Chair, Undergraduate Studies, Department of Chemical Engineering
• 2013-2020 Academic Director, WatPD Engineering

• Professional Engineers Ontario
• Canadian Society for Chemical Engineering
• Canadian Socivety for Virology

• Academic Cross-Appointment, School of Optometry, Faculty of Science, 2022 – present
• Academic Cross-Appointment, School of Pharmacy, Faculty of Science, 2020 – present
• Academic Cross-Appointment, Department of Chemistry, Faculty of Science 2012 - present
• Academic Cross-Appointment, Department of Biology, Faculty of Science 2008 – present

• CHE 180 - Chemical Engineering Design Studio 1
  • Taught in 2019, 2020, 2021, 2022
• CHE 500 - Special Topics in Chemical Engineering
  • Taught in 2020, 2021
• CHE 562 - Advanced Bioprocess Engineering
  • Taught in 2019, 2020
• CHE 660 - Principles of Biochemical Engineering
  • Taught in 2019, 2022

* Only courses taught in the past 5 years are displayed.

• Frenette A, Rodríguez-Ramos T, Zanuzzo F, Ramsay D, Semple SL, Soullière C, Rodríguez-Cornejo T, Heath G, McKenzie E, Iwanczyk J, Bruder M*, Aucoin MG, Gamperl AK, Dixon B (2022), InVitro, Ex-Vivo and In Vivo, Developmental and Comparative Immunology.
• Bruder MR* and Aucoin MG (2022), Utility of alternative promoters for foreign gene expression using the baculovirus expression vector system, Viruses.
• Bruder MR*, Aucoin MG (2022), Evaluation of virus-free manufacture of recombinant proteins using CRISPR-mediated gene disruption in baculovirus-infected insect cells, Vaccines.
• Nogueira CL*, Boegel SJ*, Shukla M, Ngo W, Jones L, Aucoin MG (2022), The Impact of a Rub and Rinse Regimen on Removal of Human Coronaviruses from Contemporary Contact Lens Materials, Contact Lens and Anterior Eye.
• Varga JFA, Brunner SR, Cheng G, Min D*, Aucoin MG, Doxey A, Dixon B. (2022), Identification and characterization of a novel peptide from rainbow trout (Oncorhynchus mykiss) with antimicrobial activity against Streptococcus iniae, Developmental and Comparative Immunology, Volume 137, Issue 104518.
• Bruder MR*, Aucoin MG (2022), Development of a sensitive assay for efficient scrutiny of AcMNPV genes using CRISPR-Cas9, Applied Microbiology and Biotechnology.
• Liu M, Bauman L, Nogueira CL*, Aucoin MG, Anderson WA, Zhao B (2022), Antimicrobial Polymeric Composites for Healthcare Applications, Current Opinion in Biomedical Engineering, Volume 22, 100395.
• Nogueira CL*, Boegel SJ*, Shukla M, Ngo W, Jones L, Aucoin MG (2022), Antiviral Activity of Contemporary Contact Lens Solutions Against Human Seasonal Coronavirus Strains, Pathogens, Volume 11, Issue 4, 472.
• Sahaa DC, Boegel SJ*, Tanvirb S, Nogueira CL*, Aucoin MG, Anderson WA, Jahed H (2022), Antiviral and antibacterial cold spray coating application on rubber substrate, disruption in disease transmission chain, Journal of Thermal Spray.
• Marcali M*, Chen X, Aucoin MG, Ren CL (2022), Droplet Formation of Biological Non-Newtonian Fluid in T-Junction Generators, Part I: Experimental Investigation, Physical Review E, Volume 105, 025105.
• Marcali M*, Chen X, Aucoin MG, Ren CL (2022), Droplet Formation with a Biological Non-Newtonian Fluid in T-Junction Generators. Part II: Model for Final Droplet Volume Prediction, Physical Review E, Volume 105, 025106.
• Sadr S*, Zargar B, Perez J, Aucoin MG, Ingalls BP (2022), Clostridium sporogenes, Frontiers in Bioengineering and Biotechnology: Synthetic Biology.
• Yip A*, Smith-Roberge J, Haghayegh Khorasania S*, Aucoin MG, Ingalls BP (2022), Calibrating Spatiotemporal Models of Microbial Communities to Microscopy Data: A Review, PLoS Computational Biology, Volume 18, Issue 10, e1010533.
• Logan M*, Rinas K, McConkey B, Aucoin MG (2022), Vero cells gain renal tubule markers in low-calcium and magnesium chemically defined media, Scientific Reports, Volume 12, 6180.
• Walji SD*, Bruder MR*, Aucoin MG (2021), Virus Matrix Interference on Virucidal Activity of High-Touch Surfaces Designed to Prevent Hospital-Acquired Infections, Antimicrobial Resistance and Infection Control, Volume 10, Issue 133, https://www.researchgate.net/publication/343011195_Virus_Matrix_Interference_on_Assessment_of_Virucidal_Activity_of_High-Touch_Surfaces_Designed_to_Prevent_Hospital-Acquired_Infections
• Quattrociocchi M*, Boegel SJ*, Aucoin MG (2021), Enhanced characterization of yeast hydrolysate combining acid digestion and 1D-1H NMR targeted profiling, Canadian Journal of Chemical Engineering, Volume 99, Issue S1, 1-11.
• Delumeau L-V, Asgarimoghaddam H, Alkie T, Jones AJB, Mistry K, Aucoin MG, DeWitte-Orr S, Musselman KP (2021), Effectiveness of Metal and Metal-Oxide Thin-Film Coatings in Decreasing the Infectious Potential of Human Coronavirus 229E, APL Materials, Volume 9, 111114.
• Havaei SM, Aucoin MG, Jahanian-Najafabadi A (2021), Pseudomonas Exotoxin based immunotoxins: over three decades of efforts on targeting cancercells with the toxin, Frontiers in Oncology: Cancer Molecular Targets and Therapeutics, Volume 11, 781800.
• Bruder MR*, Walji SD*, Aucoin MG (2021), Comparison of CRISPR-Cas9 tools for transcriptional repression and gene disruption in the BEVS, Viruses, Volume 13, Issue 1925.
• Lall D, Miscevic D, Bruder M*, Westbrook A, Aucoin M, Moo-Young M, Chou CP (2021), Escherichia coli, Bioresources and Bioprocessing, Volume 8, 122.
• Boegel SJ*, Gabriel M*, Sasges M, Petri B, Ang JC*, D’Agostina M, Zhang A, Miller M, Meunier S*, Aucoin MG (2021), Ultraviolet Disinfection of Coronaviruses: SARS CoV-2 response in-line with seasonal coronavirus surrogates, Microbiology Spectrum, Volume 9, Issue e00537-21.
• Ganobis C, Al-Abdul-Wahid MS, Renwick S, Yen S*, Carriero C, Aucoin MG, Allen-Vercoe E (2020), 1D 1H NMR as a tool for fecal metabolomics., Current Protocols in Chemical Biology, Volume 12, e83, doi: 10.1002/cpch.83
• Wulff D*, Aucoin MG, Gu FX (2020), Helium ion microscopy of corn starch, Starch, Volume 1900267, N/A, https://doi.org/10.1002/star.201900267
• Walji SD*, Aucoin MG. (2020), A Critical Evaluation of Current Protocols for Self-Sterilizing Surfaces Designed to Reduce Viral Nosocomial Infections, American Journal of Infection Control, Volume 48, Issue 10, 1255-1260, https://doi.org/10.1016/j.ajic.2020.02.008
• Moussa HI, Chan WY, Logan M*, Aucoin MG and Tsui TY (2020), Limitation in Controlling the Morphology of Mammalian Vero Cells Induced by Cell Division on Asymmetric Tungsten-Silicon Oxide Nanocomposite., Materials, Volume 13, Issue 2, 335.
• Wulff D*, Chan A, Liu Q, Gu F X, Aucoin MG (2020), Characterizing internal cavity modulation of corn starchmicrocapsules., Heliyon, Volume 6, Issue 10, e05294.
• Gabriel MA*, Dare EV*, Meunier SM*, Campbell JL, Sasges M, Aucoin MG. (2019), Ultraviolet irradiation of trypsin, lysozyme and B-galactosidase: can UVC be used as a secondary barrier against adventitious agents?, Vaccine, Volume 37, Issue 43, 6518-6525.
• Logan M*, Manalil J*, Notte C, Kearse C, George S*, Zeiser A, Farrell P, Aucoin MG (2019), A flow cytometric granularity assay for the quantification of infectious virus, Vaccine, Volume 37, Issue 47, 7090-7099.
• Shafiee F, Aucoin MG, Jahanian-Najafabadi A (2019), Targeted Diphtheria Toxin Based Therapy: A Review Article, Frontiers Microbiology, Volume 10, Issue Art. 2340, NA.
• Yen S*, Bolte E, Aucoin MG, Allen-Vercoe E (2018), Metabonomic evaluation of fecal water preparation methods: the effects of ultracentrifugation, Current Metabolomics, Volume 6, 57-63.
• Tsui TY, Logan M*, Moussa HI, Aucoin MG (2018), What's happening on the other side? Revealing nano-meter scale features of mammalian cells on engineered textured tantalum surfaces, Materials, Volume 12, Issue 1, 114.
• Moussa HI, Logan M*, Wong K, Rao Z, Aucoin MG, Tsui TY (2018), Nanoscale-textured tantalum surfaces for mammalian cell alignment., Micromachines, Volume 9, Issue 9, 464-482.
• Moussa HI, Logan M*, Chan WY, Wong K, Rao Z, Aucoin MG, Tsui TY (2018), Pattern-dependent mammalian cell (Vero) morphology on tantalum/silicon oxide 3-D nano-composites., Biomaterials: Advanced Biomaterials for Cell Adhesions, Proliferation and Differentiation, Volume 11, 1306-1322.
• Blondeel EJM*, Aucoin MG (2018), Supplementing glycosylation: A review of applying nucleotide-sugar precursors to growth medium to affect therapeutic recombinant protein glycoform distributions, Biotechnology Advances, Volume 36, Issue 5, 1505-1523.
• Dare EV*, Gabriel M*, Begum A, Sasges M, Aucoin MG (2017), The effect of hydrogen peroxide produced during ultraviolet disinfection of CHO cell culture media, Process Biochemistry, Volume 61, 147-155.
• Moussa HI, Logan M*, Siow GC, Phann DL, Aucoin MG, Tsui TY (2017), Manipulating mammalian cell morphologies using chemical-mechanical polished integrated circuit chips, Science and Technology of Advanced Materials, Volume 18, Issue 1, 839-856.
• Meunier SM*, Sasges M, Aucoin MG (2017), Evaluating Ultraviolet Sensitivity of Adventitious Agents in Biopharmaceutical Manufacturing, Journal of Industrial Microbiology & Biotechnology, Volume 44, 893-909.
• Srirangan K*, Stacey LE, Newton C, Akawi LK*, Chou CP, Aucoin MG (2017), Use of a case on metabolically engineered Escherichia coli to develop a framework for the design and analysis of bioprocesses., International Journal of Engineering Education, Volume 33, Issue 2A, 751-760.
• Blondeel EJM*, Ho R, Schulze S*, Sokolenko S*, Guillemette SR*, Slivac I, Durocher Y, Guillemette JG, McConkey BJ, Chang D, Aucoin MG (2016), An Omics Approach to Rational Feed: Enhancing Growth in CHO Cultures with NMR Metabolomics and 2D-DIGE Proteomics, Journal of Biotechnology, Volume 234, 127-138.
• Pyne ME, Sokolenko S*, Liu X, Srirangan K*, Bruder M*, Aucoin M, Moo-Young M, Chung DA, Chou CP (2016), Disruption of the reductive 1,3-propanediol pathway triggers production of 1,2-propanediol for sustained glycerol fermentation by Clostridium pasteurianum., Applied and Environmental Microbiology, Volume 82, Issue 17, 5375-5388.
• Sokolenko S*, Quattrociocchi M*, Aucoin MG (2016), Identifying model error in metabolic flux analysis - A generalized least squares approach, BMC Systems Biology, Volume 10, Issue 91, 14pp.
• Meunier SM*, Todorovic B*, Dare EV*, Begum A, Guillemette S*, Wenger A*, Saxena P, Campbell JL, Sasges M, Aucoin MG. (2016), Impact of dissolved oxygen during UV-irradiation on the chemical composition and function of CHO cell culture, PLoS ONE, Volume 11, Issue 3, e0150957.
• Thompson CM*, Aucoin MG, Kamen AA, Chapter 14: Production of Virus-Like Particles for Vaccination, Edition 3, Volume 1350, 299-314, Humana Press, 2016.
• Thompson CM*, Montes J, Aucoin MG, Kamen AA, Chapter 11: Recombinant Protein Production in Large Scale Agitated Bioreactors Using the Baculovirus Expression Vector System, Edition 3, Volume 1350, 241-261, Humana Press, 2016.
• Sokolenko S*, Aucoin M G (2015), A correction method for systematic error in 1H-NMR cell culture data validated through stochastic time-course simulation, BMC Systems Biology, Volume 9, Issue 1, 51.
• Bradley RM, Marvyn PM, Henao JJA, Mardian EB, George S*, Aucoin MG, Stark K, Duncan RD (2015), Acylglycerophosphate acyltransferase 4 (AGPAT4) is a mitochondrial lysophosphatidic acid acyltransferase that regulates brain phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol levels, Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Volume 1851, Issue 12, 1566–1576.
• Blondeel EJM*, Braasch K, McGill T*, Chang D, Spearman M, Butler M, Aucoin MG (2015), Tuning a MAb glycan profile in cell culture: Supplementing N-acetylglucosamine to favour G0 glycans without compromising productivity and cell growth., Journal of Biotechnology, Volume 214, 105-112.
• Yen S*, McDonald J, Schroeter K, Oliphant K, Sokolenko S*, Blondeel EJ*, Allen-Vercoe E, Aucoin MG (2015), Metabolomic analysis of human fecal microbiota: a comparison of feces-derived communities and defined mixed communities., Journal of Proteome Research, Volume 14, Issue 3, 1472-82.
• Thompson CM*, Petiot E, Mullick A, Aucoin MG, Henry O, Kamen AA (2015), Critical assessment of influenza VLP production in Sf9 and HEK293 expression systems, BMC Biotechnology, Volume 15, 31.
• George S*, Jauhar AM* , Mackenzie J*, Kie?lich S*, Aucoin MG (2015), Temporal characterization of protein production levels from baculovirus vectors coding for GFP and RFP genes under non-conventional promoter control, Biotechnology and Bioengineering, Volume 112, Issue 9, 1822-31.
• George S*, Aucoin MG, Characterization of Alternative Promoters toStagger and Control Protein Expression in the Baculovirus-Insect Cell System:From Intracellular Reporter Proteins to Fluorescent Influenza Virus-like Particles., 24th European Society for Animal Cell Technology (ESACT) Meeting: C2P2: Cells, Culture, Patients, Products, Barcelona, BMC Proceedings, 49-51, 2015.
• Yen S*, Sokolenko S*, Manocha B*, Blondeel EJ*, Aucoin MG, Patras A, Daynouri-Pancino F, Sasges M (2014), Treating cell culture media with UV irradiation against adventitious agents: minimal impact on CHO performance., Biotechnology Progress, Volume 30, Issue 5, 1190-5.
• Sokolenko S*, Blondeel EJ*, Azlah N*, George B*, Schulze S*, Chang D, Aucoin MG (2014), Profiling convoluted single-dimension proton NMR spectra: a Plackett-Burman approach for assessing quantification error of metabolites in complex mixtures with application to cell culture., Analytical Chemistry, Volume 86, Issue 7, 3330-7.
• Suh YH*, Kim BJ*, Tam KC, Aucoin MG (2013), Detection and characterization of hemoglobin dissociation and aggregation using microcalorimetry, Journal of Thermal Analysis and Calorimetry, Volume 115, 2159-2169.
• Nicastro J , Sheldon K , El-Zarkout F A , Sokolenko S*, Aucoin M G , Slavcev R (2013), Construction and analysis of a genetically tuneable lytic phage display system., Applied Microbiology and Biotechnology, Volume 97, Issue 17, 7791-7804.
• Srirangan K, Akawi L, Liu X, Westbrook A, Blondeel EJ*, Aucoin MG, Moo-Young M, Chou C P (2013), Manipulating the sleeping beauty mutase operon for the production of 1-propanol in engineered Escherichia coli., Biotechnology for Biofuels, Volume 6, Issue 1, 139.
• Sokolenko S*, McKay R, Blondeel E J M*, Lewis M J, Chang D, George B*, Aucoin M G (2013), Understanding the variability of compound quantification from targeted profiling metabolomics 1D-1H-NMR spectra in synthetic mixtures and urine with additional insight on choice of pulse sequences and robotic sampling, Metabolomics, Volume 9, 887–903.
• Thompson CM*, Petiot E, Aucoin MG, Henry O, Kamen AA, Developing a production process for influenza VLPs: a comparison between HEK 293SF and Sf9 production platforms., 23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health, Lille, BMC Proceedings, 22, 2013.
• Lai YP*, Sun KEK*, Takamura H*, Moresoli C, Aucoin MG, Designing an undergraduate food processing laboratory for the University of Waterloo, Canadian Engineering Education Association (ceea13) Conference, 2013.
• Sokolenko S*, Nicastro J, Slavcev R, Aucoin M G (2012), Graphical analysis of flow cytometer data for characterizing controlled fluorescent protein display on ? phage., Cytometry. Part A : The Journal of the International Society for Analytical Cytology, Volume 81, Issue 12, 1031-1039.
• George S*, Sokolenko S*, Aucoin M G (2012), Rapid and cost-effective baculovirus sample preparation method as a viable alternative to conventional preparation for quantitative real-time PCR., Journal of Virological Methods, Volume 182, Issue 1-2, 27-36.
• Sokolenko S*, George S*, Wagner A*, Tuladhar A*, Andrich J M*, Aucoin M G (2012), Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks., Biotechnology Advances, Volume 30, Issue 3, 766-781.
• Moonesan MS, George S*, Aucoin M, Jayaram S, Effect of process parameters on the inactivation and electrode reactions during PEF treatment of liquid foods, International Conference Bio & Food Electrotechnologies, BFE 2012, Salerno, 2012.
• Kamen A A, Aucoin M G, Merten O W, Alves P, Hashimoto Y, Airenne K, Hu Y C, Mezzina M, van Oers M M (2011), An initiative to manufacture and characterize baculovirus reference material., Journal of Invertebrate Pathology, Volume 107 Suppl, 113-117.
• Transfiguracion J , Mena J A , Aucoin M G , Kamen A A (2011), Development and validation of a HPLC method for the quantification of baculovirus particles., Journal of Chromatography B,, Volume 879, Issue 1, 61-68.
• Sokolenko S*, Cheng Y-L*, Aucoin M G (2010), Getting more from cell size distributions: establishing more accurate biovolumes byestimating viable cell populations, Biotechnology Progress.
• Mena JA, Aucoin MG, Montes J, Chahal PS, Kamen AA (2010), Improving adeno-associated vector yield in high density insect cell cultures, Journal of Gene Medicine.
• Aucoin M G, Mena J A , Kamen A A (2010), Bioprocessing of baculovirus vectors: a review, Current Gene Therapy, Volume 10, Issue 3, 174-186.

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