You are here

Bayesian-based deconvolution fluorescence microscopy using dynamically updated nonparametric nonstationary expectation estimates

TitleBayesian-based deconvolution fluorescence microscopy using dynamically updated nonparametric nonstationary expectation estimates
Publication TypeJournal Article
Year of Publication2015
AuthorsWong, A., X. Wang, and M. Gorbet
JournalNature Scientific Reports
Abstract

Fluorescence microscopy is widely used for the study of biological specimens. Deconvolution can significantly improve the resolution and contrast of images produced using fluorescence microscopy; in particular, Bayesian-based methods have become very popular in deconvolution fluorescence microscopy. An ongoing challenge with Bayesian-based methods is in dealing with the presence of noise in low SNR imaging conditions. In this study, we present a Bayesian-based method for performing deconvolution using dynamically updated nonstationary expectation estimates that can improve the fluorescence microscopy image quality in the presence of noise, without explicit use of spatial regularization.

DOI10.1038%2Fsrep10849