Thursday
October
24,
2013
4:00
pm,
PHYS
235
"Seeing
cells
in
the
living
eye:
Pushing
the
limits
of
high-resolution
retinal
imaging"
Dr.
Jennifer
Hunter
Assistant
Professor,
Departments
of
Ophthalmology,
Biomedical
Engineering
and
Center
for
Visual
Science
University
of
Rochester
Abstract:
Visualization
of
individual
cells
in
the
living
retina
is
critical
to
understanding
normal
retinal
structure
and
its
changes
with
disease.
Advances
in
adaptive
optics
scanning
laser
ophthalmoscopy
have
made
it
possible
to
image
the
living
retina
with
better
resolution
than
ever
before,
making
it
possible
to
see
the
smallest
retinal
cells
including
cones
at
the
foveal
center,
individual
rods,
and
blood
cells
flowing
through
the
smallest
capillaries.
Other
cell
layers
in
the
retina
are
either
transparent
or
opaque
and
are
thus
more
difficult
to
visualize.
However,
the
addition
of
fluorescence
imaging
capabilities
is
making
it
possible
to
successfully
image
a
larger
variety
of
cells.
With
single
photon-fluorescence,
the
retinal
pigment
epithelium,
a
single
layer
of
opaque
cells
behind
the
cones
that
provide
critical
support
for
photoreceptors,
is
now
accessible.
By
using
extrinsic
fluorophores,
the
structure
and
function
of
transparent
neural
cells
can
be
observed.
Two-photon
fluorescence
imaging
in
the
living
eye
provides
a
method
to
non-invasively,
without
the
use
of
extrinsic
fluorophores,
image
not
only
retinal
structure,
but
also
to
assess
retinal
function.
Please
contact
Melanie
Campbell
if
you
are
interested
in
meeting
with
Dr.
Hunter
on
Oct
24
or
25.