This work presents an evaluation of solid-phase microextraction (SPME) SPME in combination with liquid chromatography-high resolution mass spectrometry (LC-HRMS) as an analytical approach for untargeted brain analysis. The study included a characterization of the metabolite coverage provided by C18, mixed-mode (MM, with benzene sulfonic acid and C18 functionalities), and hydrophilic lipophilic balanced (HLB) particles as sorbents in SPME coatings after extraction from cow brain homogenate at static conditions.
The present work documents the development and validation of a portable fluorometer designed to detect and quantify the fluorescence of DOX that has been extracted using SPME probes. This project is a continuation of our pioneering works on in vivo SPME.
The objective of this work was to introduce in vivo SPME sampling as a new method to accurately measure detailed oxylipin profiles in the brains of living, awake rats.
