Thursday
October
24,
2013
1:30
pm,
E5-3052
Bio-MEMS
Seminar:
"High-Throughput
Analysis
of
Protein-Protein
Interactions
Using
Droplet-based
Microfluidics"
Dr.
Soo-Ik
Chang
Professor
of
Biochemistry,
Chungbuk
National
University
Abstract:
In
recent
years
droplet-based
(or
segmented-flow)
microfluidic
systems
have
emerged
as
a
powerful
technological
platform
for
performing
high-throughput
chemical
and
biological
experimentation.
Since
droplets
may
be
generated
at
kHz
frequencies,
enormous
experimental
data
sets
can
also
be
amassed
in
short
times
allowing
complex
statistical
analyses
of
multi-component
systems.
Whilst
droplet-based
microfluidic
systems
are
efficient
in
generating
vast
amounts
of
chemical
and
biological
information,
the
development
of
sensitive
detection
methods
is
critical
in
allowing
this
information
to
be
extracted
and
utilized.
To
this
end,
a
variety
of
optical
detection
methods
for
probing
segmented
flows
in
real
time
have
been
developed.
I
will
present
the
use
of
fluorescence
resonance
energy
transfer
(FRET)
and
fluorescence
polarization
(FP)
detections
in
a
droplet-based
microfluidic
system.
Specifically,
I
will
present
protein-protein
interactions
between
angiogenin,
a
small
polypeptide
implicated
in
angiogenesis
and
in
tumor
growth,
and
an
anti-angiogenin
antibody
in
a
pico-liter
droplet
by
using
FRET
and
FP
detections
To
meet
with
Dr.
Chang
please
email
Carolyn
Ren.