Designed DNA Nano-switches as Sensitive Electrochemical Biosensors
Dr.
Hua-Zhong
"Hogan"
Yu
Department
of
Chemistry
Simon
Fraser
University
Burnaby,
British
Columbia
Monday,
December
5,
2022
11:00
a.m.
In-person:
C2-
361
(Reading
Room)
and
Online
via
MS
Teams (please
email
Victoria
Van
Cappellen
at vvancapp@uwaterloo.ca for
access)
Abstract: Functional nucleic acid receptors (aptamers) have emerged as effective and robust recognition elements for state-of-the-art biosensors. Analytical readouts from aptamer-based biosensors (whether optical, electrochemical, or otherwise) derive primarily from global-scale conformational changes induced in the aptamer domain by analyte binding. Herein, we describe a unique biosensor design principle that represents a distinct alternative to this paradigm; particularly we demonstrate the ready applicability of this design principle in the de novo creation of electrochemical sensors for a clinical analyte of current interest. The function of the class of biosensors we describe, termed “DNA nano-switches”, is designed to depend on the integrity of duplex DNA-mediated charge transfer between an electrode and a redox label. Our recent selection of DNAzymes for the Nobel winning “click” reaction” will be also briefly presented.
[1] Tang, Y.; Ge, B.; Sen, D.; Yu, H.-Z. Chem. Soc. Rev. 2014, 43, 518–529 (Tutorial Review) [2] Ma, F.; Qi, L.; Einarson, O.; Sen, D.; Yu, H.-Z. Anal. Chem. 2019, 91, 8244−8251. [3] Liu, K.; Lat, P. K.; Yu, H.-Z.; Sen, D. Nucleic Acid Res. 2000, 48, 7356–7370.
