Recent development and applications in U-shaped mobility analyzer - Mass spectrometry
Dr. Wenjian Sun
Managing Director
Shimadzu Research Laboratory, Shanghai, China
Friday, July 26, 2024
2:30 p.m.
In-person: C2-361 (Reading Room)
Abstract: U-shaped mobility analyzer is a new type of counter-flow ion mobility spectrometers in which ions can be separated based on their mobility along a U-shaped trajectory. One unique feature of UMA is that it can be operated as a high-resolution mobility filter, which enables high dynamic range and good quantitation capability. This makes UMA an ideal device for coupling either to a triple quadrupole MS (QqQ) or a quadrupole time-of-flight MS (qTOF) with high flexibility for various qualitative and quantitative applications. Mobility resolution of UMA can reach 100~200 under typical instrument settings. By slowing down the scan speed and increasing the gas flow rate, the resolution can be >200 for small molecules, and >400 for proteins. Other features of UMA include fast drift gas switching, tandem mobility function, and wide mobility range. One of its important applications for lipid isomer analysis will be emphasized, in which a special lipid derivatization method (Aza Prilezhaev aziridination, APA) coupled with the unique pseudo MS3 function of UMA makes it possible for identifying multiple levels of unsaturated lipid isomerization with high efficiency.