Guy Guillemette

• Protein structure and function
• nitric oxide synthase
• calmodulin
• enzymology
• biophysics

Nitric Oxide (NO) is an important regulator of numerous essential physiological processes like blood pressure, neurotransmission, insulin release, memory storage and the immune response. In addition, NO is involved in a variety of serious pathophysiological processes including septic shock, atherosclerosis, diabetes, as well as myocardial and cerebral ischemia. The biogenesis of NO is catalyzed by Nitric Oxide Synthase (NOS) enzymes. The long-term goal of our research program is to understand the regulation and mechanism of the NOS enzymes.

Guy Guillemette teaches both undergraduate and graduate courses. Course offerings have included topics in biochemistry, biomaterials, and metalloproteins and metalloenzymes.

Recent publications include

• Pimlott, Douglas, Guy Guillemette, and Thorsten Dieckmann. "Structural Investigation of Melatonin Metabolites as Calmodulin Antagonists and potential Nitric Oxide Synthase Inhibitors." The FASEB Journal 33.1_supplement (2019): 784-12.
• Piazza M., Dieckmann T., Guillemette J.G. (2018). Investigation of the structure and dynamic of calmodulin-nitric oxide synthase complexes using NMR spectroscopy. Front. Biosci. 23, 1902-1922. 
• Piazza M, Taiakina V., Dieckmann T, Guillemette JG (2017). Structural consequences of Calmodulin EF muations. Biochemistry 56, 944-956.  
• Piazza, M., Dieckmann, T., Guillemette, J.G. (2016). StructuralStudies of a Complex between endothelial Nitric Oxide Synthase and Calmodulin at Physiological Calcium Concentration. Biochemistry 55, 5962- 5971.
• Blondeel, E.J.M., Ho, R., Schulze, S., Sokolenko,, S., Guillemette, S., Slivac, I., Durocher, Y., Guillemette, J.G., McConkey, B., Chang, D., Aucoin, M.G. (2016). An Omics Approach to Rational Feed: Enhancing Growth in CHO Cultures with NMR Metabolomics and 2D-DIGE Proteomics Journal of Biotechnology. Journal of Biotechnology 234, 127-138.
• Piazza, M., Guillemette. J.G., Dieckmann T. (2016). Chemical shift assignments of calmodulin constructs with EF hand mutations. Biomol. NMR Assign. 10, 193-198.
• Piazza, M., Guillemette, J.G., Dieckmann, T. (2015) Chemical shift perturbations induced by residue specific mutations of CaM interacting with NOS peptides. Biomolecular NMR Assigments 9(2) 299-302.

Patent Application – “Spatio-Temporal Activation, Site Selection, and Monitoring of Treatment Agent and Activity Thereof  Using EMR”, Jervis E.J., Guillemette J.G., Perdicakis B., Montgomery H.J., Lajoie G.A. Fishlock D., US and European Patent Applications Filed Oct 12, 2005.

• FRSQ Chercheur-Boursier ($43,000/yr towards salary) Investigation of the function of the pyruvate dehydrogenase enzyme. Declined after June 30, 1995, 1993-1997

• Scholarship from the Huntington’s Society of Canada, 1986   

• OGS scholarship, 1985

• Scholarship from the Huntington’s Society of Canada, 1984

• Member of the Institute of Biochemistry & Molecular Biology

• Editorial Board member, Biochemical Research Letters, 2009-present
• Journal of Biomedicine and Biotechnology, 2008-present
• Guelph-Waterloo Centre for Graduate Work in Chemistry and Biochemistry (GWC)² Director, 2012-2015
• Science Faculty Council, 2012-2015
• Chemistry Safety committee, 2015
• Graduate Officer, 2009-2012
• IBMB Director, 2009-2012
• Graduate advisor committee, 2009-2012
• (GWC)² Coordinating Committee, 2005-2012
• Undergraduate Relations Committee, 2003-2012

1988 PhD, Biochemistry, University of Toronto

1981 BSc, Biochemistry, University of Toronto