Janusz Pawliszyn

Janusz Pawliszyn
University Professor, Canada Research Chair, NSERC Industrial Research Chair in New Analytical Methods and Technologies
Location: C2 278A
Phone: 519-888-4567 x84641


The primary focus of Janusz Pawliszyn's research program is the design of highly automated and integrated instrumentation for the isolation of analytes from complex matrices and the subsequent separation, identification and determination of these species.

The primary separation tools used by his group are Gas Chromatography, Liquid Chromatography and Capillary Electrophoresis coupled to variety of detections systems, including a range of mass spectrometry techniques.

Currently his research is focusing on the elimination of organic solvents from the sample preparation step to facilitate on-site monitoring and in-vivo analysis. Several alternative techniques to solvent extraction are investigated including the use of coated fibers, packed needles, membranes and supercritical fluids.

Janusz Pawliszyn is exploring the application of the computational and modeling techniques to enhance performance of sample preparation, chromatographic separations and detection. The major area of his interest involves the development and application of imaging detection techniques for microcolumn chromatography, capillary electrophoresis and micro chip separation devices.

Research Interests

  • Solid Phase Microextraction (SPME)
  • BioSPME
  • SPME Cold Fibre
  • Thin Film Membranes
  • Thin Film Brushes
  • Thin Film and SPME Fibre
  • Needle Trap, Thin Film and SPME
  • Devices and Analytical Methods


  • 1982 Ph.D., Analytical Chemistry, Southern Illinois University, USA
  • 1978 M.Sc., Organic Chemistry, Technical University of Gdansk, Poland
  • 1977 B.Sc., Engineering, Technical University of Gdansk, Poland


  • 2023 A.J.P. Martin Medal, Chromatographic Society
  • 2023 The CIC Medal, Chemical Institute of Canada
  • 2019 Top 10 influential persons in analytical science globally Analytical Scientist’s 2019 Power List, Analytical Scientist
  • 2019 Talanta Medal, Talanta, Elsevier
  • 2018 ACS Award in Chromatography, American Chemical Society
  • 2017 Pittsburgh Conference Analytical Chemistry Award
  • 2015 Halász Medal Award, The Hungarian Society for Separation Sciences
  • 2015 Maria Skłodowska-Curie Medal, Polish Chemical Society
  • 2013 LeSueur Memorial Award, Society of Chemical Industry (SCI)
  • 2013 Environmental Research and Development Award
  • 2012 E.W.R. Steacie Award, Chemical Institute of Canada
  • 2012-14 Visiting Professor, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, China
  • 2012 Associate Editor of Journal of Pharmaceutical Analysis
  • 2011 PittCon Dal Nogare Award, Pittsburgh Conference Analytical Chemistry
  • 2010 Fellow of Royal Society of Canada
  • 2010 Member of Review Committee for University of Amsterdam Chemistry Programs for Quality Assurance Netherlands Universities
  • 2010 Ontario Premier’s Innovation Award
  • 2010 University Professor, University of Waterloo
  • 2010 ACS Award in Separation Science and Technology, American Chemical Society
  • 2010 Marcel Golay Award
  • 2010 Torbern Bergman Medal, The Swedish Chemical Society


  • Member of the Waterloo Institute for Nanotechnology (WIN) and The Water Institute
  • Canada Research Chair, 2003-present
  • NSERC Industrial Research Chair in New Analytical Methods and Technologies, 1994-present
  • Editor in Chief for Trends in Analytical Chemistry (TrAC), 2015-present
  • Editor in Chief for Green Analytical Chemistry, 2021-present
  • Editor of Analytica Chemica Acta, 2007-present
  • Associate Editor of Journal of Pharmaceutical Analysis, 2011-2021
  • IUPAC Orange Book, Extraction Chapter, 2012-15
  • Member of the College of Reviewers at CFI, 2003-15
  • Editorial Board Member of the Journal of Microcolumn Separations, 1991-2015
  • Editor in Chief of Comprehensive Sampling and Sample Preparation (Elsevier), 2009-14
  • Advanced Health Technologies Panel, ORF, 2012-13
  • NSERC DG Evaluation Team, 2009-13
  • Editorial Board Member of Chemia Analityczna, 2008-09

Affiliations and Volunteer Work

  • Member, The Water Institute
  • Member, Waterloo Institute for Nanotechnology


  • CHEM 400 - Special Topics in Chemistry
    • Taught in 2022, 2023
  • CHEM 727 - Separations
    • Taught in 2021, 2023

* Only courses taught in the past 5 years are displayed.

Selected/Recent Publications

  • H. Rosales-Solano, V. Galievsky, K. Murtada, P. Radovanovic, and J. Pawliszyn. Profiling of Unsaturated Lipids by Raman Spectroscopy Directly on Solid-Phase Microextraction Probes. Anal. Chem. 94, 606–611 (2022).
  • A. Kasperkiewicz, S. Lendor and J. Pawliszyn. Impact of pesticide formulation excipients and employed analytical approach on relative matrix effects of pesticide determination in strawberries. Talanta 236, Article #122825 (2022).
  • E. Yiantzi, K. Murtada, K. Terzidis, J. Pawliszyn and E. Psillakis. Vacuum-assisted headspace thin-film microextraction: Theoretical formulation and method optimization for the extraction of polycyclic aromatic hydrocarbons from water samples. Analytica Chimica Acta, 1186, 339217 (2022).
  • S. Zeinali, M. Wieczorek and J. Pawliszyn. Free versus droplet-bound aroma compounds in sparkling beverages. Food Chemistry 378, Article #131985 (2022).
  • J. Yu, X. Xu, K. Murtada and J. Pawliszyn. Untargeted analysis of microbial metabolites and unsaturated fatty acids in salmon via hydrophilic-lipophilic balanced solid-phase microextraction arrow. Food Chemistry 380, Article #132219 (2022).
  • M. Huq, H. Rosales-Solano and J. Pawliszyn. Investigation of binding of fatty acids to serum albumin to determine free concentrations: Experimental and in-silico approaches. Analytica Chimica Acta, 1192, 339370 (2022).
  • J. Wu, W. McElroy, J. Pawliszyn, C. Heger. Imaged capillary isoelectric focusing: Applications in the pharmaceutical industry and recent innovations of the technology. Trends in Analytical Chemistry 150, Article #116567 (2022).
  • Shakiba Zeinali, Chiranjit Ghosh and J. Pawliszyn. Simultaneous determination of exhaled breath vapor and exhaled breath aerosol using filter-incorporated needle-trap devices: A comparison of gas-phase and droplet-bound components. Analytica Chimica Acta, 1203, 339671 (2022).
  • Shakiba Zeinali and J. Pawliszyn. Effect of household air pollutants on the composition of exhaled breath characterized by solid-phase microextraction and needle-trap devices. Analytical and Bioanalytical Chemistry, https://doi.org/10.1007/s00216-022-03997-6 (2022).
  • Shakiba Zeinali and J. Pawliszyn. Green Portable Method for Simultaneous Investigation of Gaseous and Particle-Bound Air Pollutants in Indoor and Outdoor Environments. ACS Sustainable Chemistry & Engineering, 10, 3981-3989 (2022).


  • "Method and Apparatus for Detecting Universally and Selectively Concentration Gradients and for Deflecting a Light Beam in a Controlled Fashion", U.S. Pat. 4,784,494 (issued November 15, 1988); 4,940,333 ((issued July 10, 1990); 4,993,832 (issued February 19, 1991); 5,153,666 (issued October 6, 1993).
  • "Apparatus and Method for Delivering Supercritical Fluids", U.S. Pat. 5,237,824 (issued August 24, 1993).
  • "Apparatus for performing and universally detecting capillary isoelectric focusing without mobilization using concentration gradient imaging systems" US Pat. 5,395,502 (March 7, 1995).
  • "A Device and Process for Increasing Analyte Concentration in a Sorbent" U.S. Pat. 5,496,741 (issued March 5, 1996).
  • "Process and Device for Continuous Extraction and Analysis of Fluid Using Membrane" U.S. Pat. 5,492,838 (issued February 20, 1996).
  • "Method and Device for Solid Phase Microextraction and Desorption", U.S. Pat. 5,691, 206 (issued November 25, 1997); 6,042,787 (issued March 28, 2000); 7,674,631 (March 9, 2010) and European and Japanese counterparts.
  • "Electrophoresis separation in a capillary passage" US Pat. 5,784,154 (issued July 21, 1998)
  • “Method and Device for Isoelectric Focusing without Carrier Ampholytes” U.S. Pat. 5,759,370 (issued June 2, 1998).
  • “Fast Sampling Device and Sampling Method for Capillary Electrophoresis” US Pat. 5,985,121 (issued Nov. 16, 1999).
  • “Needle Trap” US Pat. 6,481,301 (issued Nov. 19, 2002).
  • “Analytical devices based on diffusion boundary layer calibration and quantitative sorption” US Pat. 6,588,255 (issued July 8, 2003) and 6,941,825 (issued September 13, 2005).
  • “Measurement of fluorescence using capillary isoelectric focusing” US Pat. 6,852,206 (issued Feb. 8, 2005).
  • “Calibration procedure for investigating biological systems” US Pat. 7,232,689 (issued June 19, 2007), 8,008,064 (issued August 30, 2011), 8,080,407 (issued December 20, 2011), 8,114,660 (issued February 14, 2012),.
  • “Multiple sampling device and method for investigating biological systems” US Pat. 7,259,019 (issued August 21, 2007).
  • “Micro-devices and analytical procedures for investigation of biological systems” US Pat 7,384,794 (issued June 10, 2008) and European and Japanese counterparts.
  • “Device and method for micro sorbent extraction and desorption” US Pat 8,104,331 (issued January 31, 2012); 7,645,611 (issued January 12, 2010) and European and Japanese counterparts.
  • “Solid-phase microextraction coatings and methods for their preparation” US Pat 7,479,390 (issued December 3, 2013).
  • “Standard Analyte Generator” US Pat 9,625,426 (issued April 18, 2017)
  • “Probe for extraction of molecules of interest from a sample” US Pat 9,870,907 (issued January 16th, 2018)
  • “Method for measuring or identifying a component of interest in a biological system” US Pat 9,891,150 (issued Feb 13th, 2018)
  • “System and method for desorbing and detecting an analyte sorbed on a solid phase microextraction device” US Pat 10,393,636 (issued 2019/8/27)
  • “System and method for desorbing and detecting an analyte sorbed on a solid phase microextraction device” US Pat 10,429,362 (issued 2019/10/1)
  • “Method and instrument for extracting a component from a sample” US Pat 10,545,073 (issued January 28, 2020)
  • “Solid phase microextraction coating’ US Pat 10,545,077 (issued January 28, 2020)
  • “Ion Mobility Spectrometer and Method of Ionizing the Ions” US Pat 11, 275,054 (issued March 15, 2022)

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